Bone Marrow-Derived Mononuclear Cells Promote Improvement in Glomerular Function in Rats with Early Diabetic Nephropathy

被引：14

作者：

Castiglione, Raquel C. ^{[1
]}

Maron-Gutierrez, Tatiana ^{[1
,2
]}

Barbosa, Carolina M. L. ^{[1
]}

Ornellas, Felipe M. ^{[1
]}

Barreira, Andre Luis ^{[3
]}

diBarros, Carolina B. A. ^{[4
]}

Vasconcelos-dos-Santos, Andreia ^{[5
]}

Paredes, Bruno Diaz ^{[6
]}

Pascarelli, Bernardo M. ^{[7
]}

Diaz, Bruno L. ^{[8
]}

Rossi-Bergmann, Bartira

Takiya, Christina M.

Rocco, Patricia R. M. ^{[2
]}

Souza-Menezes, Jackson ^{[9
]}

Morales, Marcelo M. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Celular & Mol, BR-21941902 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Invest Pulm, BR-21941902 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Lab Patol Celular, Inst Biofis Carlos Chagas Filho, BR-21941902 Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Imunofarmacol, BR-21941902 Rio De Janeiro, Brazil

[5] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Neurobiol Celular & Mol, BR-21941902 Rio De Janeiro, Brazil

[6] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Cardiol Celular & Mol, BR-21941902 Rio De Janeiro, Brazil

[7] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Patol, Rio De Janeiro, Brazil

[8] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Inflamacao, BR-21941902 Rio De Janeiro, Brazil

[9] Univ Fed Rio de Janeiro, Lab Bioquim Integrada Hatisaburo Masuda Nucl Ecol, BR-27971220 Macae, RJ, Brazil

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2013年 / 32卷 / 03期

关键词：

Bone marrow mononuclear cells; Diabetic nephropathy; Renal function; Arginase I plus macrophages; Cytokines; GROWTH-FACTOR-BETA; KIDNEY-DISEASE; STROMAL CELLS; MACROPHAGE ACTIVATION; EXTRACELLULAR-MATRIX; TGF-BETA; ALTERNATIVE PATHWAY; INJURY; TRANSPLANTATION; HYPERGLYCEMIA;

D O I：

10.1159/000354473

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Background/Aims: Diabetic nephropathy is one of the main causes of end- stage renal disease. The present study investigated the effect of mononuclear cell (MC) therapy in rats subjected to diabetic nephropathy. Methods: Male Wistar rats were divided into control (CTRL), diabetic (DM), CTRL+MC and DM+MC groups. Diabetes was induced by a single injection of streptozotocin (45 mg/kg, i.p.) and, 4 weeks later, 2x10(7) MCs were injected via the jugular vein. Results: The rats in the DM and DM+MC groups showed increased glycemia, glomerular filtration rate and glomerular tuff area versus control groups. The glomerular filtration rate and glomerular tuff area were normalized in the DM+MC group. No alterations were observed in the fractional excretion of electrolytes and proteinuria between the DM and DM+MC groups. TGF-beta 1 protein levels in the DM group were significantly increased versus control animals and normalized in the DM+MC group. An increase in ED1+/arginase I(+)macrophages and IL-10 renal expression was observed in the DM+MC group versus DM group. Conclusions: Bone marrow- derived MC therapy was able to prevent glomerular alterations and TGF-beta 1 protein overexpression and modulated glomerular arginase I+ macrophage infiltration in rats subjected to early diabetic nephropathy. Copyright (C) 2013 S. Karger AG, Basel

引用

页码：699 / 718

页数：20

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