Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

被引：130

作者：

Zerbe, Philipp ^{[1
]}

Bohlmann, Joerg ^{[2
]}

机构：

[1] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA

[2] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

来源：

TRENDS IN BIOTECHNOLOGY | 2015年 / 33卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

diterpene synthase; plant chemical diversity; synthetic biology; metabolic engineering; natural product biosynthesis; ENT-COPALYL DIPHOSPHATE; SINGLE RESIDUE SWITCH; FUNCTIONAL-CHARACTERIZATION; ABIETADIENE SYNTHASE; TERPENE SYNTHASES; KAURENE SYNTHASE; SPECIALIZED METABOLISM; NETWORK ANALYSIS; GENE DISCOVERY; BIOSYNTHESIS;

D O I：

10.1016/j.tibtech.2015.04.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plants produce thousands of diterpenoid natural products; some of which are of significant industrial value as biobased pharmaceuticals (taxol), fragrances (sclareol), food additives (steviosides), and commodity chemicals (diterpene, resin acids). In nature, diterpene synthase (diTPS) enzymes are essential for generating diverse diterpene hydrocarbon scaffolds. While some diTPSs also form oxygenated compounds, more commonly, oxygenation is achieved by cytochrome P450-dependent mono-oxygenases. Recent genome-, transcriptome-, and metabolome-guided gene discovery and enzyme characterization identified novel diTPS functions that form the core of complex modular pathway systems. Insights into diterpene metabolism may translate into the development of new bioengineered microbial and plant-based production systems.

引用

页码：419 / 428

页数：10

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