The human mitochondrial elongation factor tu (EF-Tu) gene: cDNA sequence, genomic localization, genomic structure, and identification of a pseudogene

被引:37
作者
Ling, MF
Merante, F
Chen, HS
Duff, C
Duncan, AMV
Robinson, BH
机构
[1] HOSP SICK CHILDREN, DEPT GENET, TORONTO, ON M5G 1X8, CANADA
[2] UNIV TORONTO, DEPT BIOCHEM, TORONTO, ON, CANADA
[3] QUEENS UNIV, DEPT PATHOL, KINGSTON, ON K7L 2V7, CANADA
[4] KINGSTON GEN HOSP, KINGSTON, ON K7L 2V7, CANADA
基金
英国医学研究理事会;
关键词
nuclear encoded; Northern blot; pseudogene;
D O I
10.1016/S0378-1119(97)00279-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The human mitochondrial elongation factor Tu (EF-Tu) is nuclear-encoded and functions in the translational apparatus of mitochondria. The complete human EF-Tu cDNA sequence of 1677 base pairs (bp) with a 101 bp 5'-untranslated region, a 1368 bp coding region, and a 207 bp 3'-untranslated region, has been determined and updated. The predicted protein from this cDNA sequence is similar to 49.8 kDa in size and is composed of 455 amino acids (aa) with a putative N-terminal mitochondrial leader sequence of similar to 50 aa residues. The predicted amino acid sequence shows high similarity to other EF-Tu protein sequences from ox, yeast, and bacteria, and also shows limited similarity to human cystolic elongation factor 1 alpha. The complete size of this cDNA (1677 bp) obtained by cloning and sequencing was confirmed by Northern blot analysis, which showed a single transcript (mRNA) of similar to 1.7 kb in human liver. The genomic structure of this EF-Tu gene has been determined for the first time. This gene contains nine introns with a predicted size of similar to 3.6 kilobases (kb) and has been mapped to chromosome 16p11.2. In addition, an intronless pseudogene of similar to 1.7 kb with 92.6% nucleotide sequence similarity to the EF-Tu gene has also been identified and mapped to chromosome 17q11.2. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:325 / 336
页数:12
相关论文
共 38 条
[21]   Protein synthesis - An elongation factor turn-on [J].
Nierhaus, KH .
NATURE, 1996, 379 (6565) :491-492
[22]  
NOER AS, 1991, AM J HUM GENET, V49, P715
[23]   MITOCHONDRIAL POLYPEPTIDE ELONGATION-FACTOR EF-TU OF SACCHAROMYCES-CEREVISIAE - FUNCTIONAL AND STRUCTURAL HOMOLOGIES TO ESCHERICHIA-COLI EF-TU [J].
PIECHULLA, B ;
KUNTZEL, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 132 (02) :235-240
[24]   Familial cardiomyopathy with cataracts and lactic acidosis: A defect in complex I (NADH-Dehydrogenase) of the mitochondria respiratory chain [J].
Pitkanen, S ;
Merante, F ;
McLeod, DR ;
Applegarth, D ;
Tong, T ;
Robinson, BH .
PEDIATRIC RESEARCH, 1996, 39 (03) :513-521
[25]  
ROSENTHAL LP, 1987, J BIOL CHEM, V262, P10955
[26]  
Sambrook J., 1989, MOL CLONING LAB MANU
[27]  
SCHNEPF E, 1971, P299
[28]  
SCHWARTZBACH CJ, 1991, J BIOL CHEM, V266, P16324
[29]  
SCHWARTZBACH CJ, 1989, J BIOL CHEM, V264, P19125
[30]   A SINGLE AMINO-ACID SUBSTITUTION IN ELONGATION FACTOR-TU DISRUPTS INTERACTION BETWEEN THE TERNARY COMPLEX AND THE RIBOSOME [J].
TUBULEKAS, I ;
HUGHES, D .
JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :240-250