Melting curve analysis in a snapshot

被引：28

作者：

Baaske, Philipp ^{[1
]}

Duhr, Stefan ^{[1
]}

Braun, Dieter ^{[1
]}

机构：

[1] Univ Munich, Dept Appl Phys, D-80799 Munich, Germany

来源：

APPLIED PHYSICS LETTERS | 2007年 / 91卷 / 13期

关键词：

D O I：

10.1063/1.2790806

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The thermal denaturation of molecules is an essential method in biochemistry and diagnostics, including the measurement of single nucleotide polymorphisms and the binding analysis of proteins. We present a method for the all-optical high speed measurement of melting curves. A thin sheet of water is locally heated with an infrared laser to obtain a spatial temperature distribution between 20 and 100 degrees C. Using a fluorescence microscope a melting curve is recorded within 50 ms. This is about 10 000-times faster than state-of-the-art fluorometry and yields the same results for the validation example of a DNA hairpin. (c) 2007 American Institute of Physics.

引用

页数：3

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