Gene cloning and characterization of VcrM, a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1

被引：49

作者：

Huda, MN ^{[1
]}

Chen, J ^{[1
]}

Morita, Y ^{[1
]}

Kuroda, T ^{[1
]}

Mizushima, T ^{[1
]}

Tsuchiya, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Dept Microbiol, Okayama 7008530, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 47卷 / 06期

关键词：

multidrug resistance; drug efflux pump; VcrM; Vibrio cholerae non-O1;

D O I：

10.1111/j.1348-0421.2003.tb03379.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We cloned a DNA fragment responsible for drug resistance from chromosome of Vibrio cholerae non-O1. Nucleotide sequence analysis of this fragment revealed the presence of a single open reading frame encoding a protein consisting of 445 amino acid residues. We designated the gene as vcrM. Hydropathy analysis of the deduced amino acid sequence of VcrM suggests the presence of 12 trans-membrane segments. A dendrogram showed that VcrM is a member of the DinF-subfamily within the MATE family of multidrug efflux pumps. Expression of the cloned vcrM gene in drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4', 6-diamidino-2-phenylindole, Hoechst 33342, rhodamine 6G, tetraphenylphosphonium chloride (TPPCI) and ethidium bromide. Efflux of acriflavine due to VcrM was dependent on Na+ or Li+. Moreover, Na+ efflux was observed with VcrM when TPPCI was added to Na+-loaded cells. Therefore, we conclude that VcrM is a Na+/drug antiporter-type multidrug efflux pump.

引用

页码：419 / 427

页数：9

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