A globin in the nucleus!

被引：134

作者：

Geuens, E ^{[1
]}

Brouns, I ^{[1
]}

Flamez, D ^{[1
]}

Dewilde, S ^{[1
]}

Timmermans, JP ^{[1
]}

Moens, L ^{[1
]}

机构：

[1] Univ Antwerp, Dept Biomed Sci, B-2610 Antwerp, Belgium

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 33期

关键词：

D O I：

10.1074/jbc.C300203200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytoglobin and neuroglobin are recently discovered members of the globin family. In situ hybridization localized neuroglobin mainly in brain and retina, while cytoglobin was expressed ubiquitously in all analyzed tissues. In the present study, polyclonal antibodies were raised against both proteins and the distribution of them was studied by immunocytochemistry at tissue and subcellular level. Cytoglobin immunoreactivity was uniformly distributed and found in all tissues studied. At the subcellular level, cytoglobin immunoreactivity was exclusively detected in the cell nucleus. In contrast, neuroglobin immunoreactivity was detected in specific brain regions with varying intensities and in the islet of Langerhans in the pancreas. The immunoreactivity was restricted to the cytoplasm of neurons and endocrine beta cells. The nuclear localization of cytoglobin opens new perspectives for possible function(s) of globin-folded proteins as transcriptional regulators.

引用

页码：30417 / 30420

页数：4

共 47 条

[1] Characterization of human stellate cell activation-associated protein and its expression in human liver [J].

Asahina, K ;

Kawada, N ;

Kristensen, DB ;

Nakatani, K ;

Seki, S ;

Shiokawa, M ;

Tateno, C ;

Obara, M ;

Yoshizato, K .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (03) :471-475

[2] ENDOCRINE PANCREAS - 3-DIMENSIONAL RECONSTRUCTION SHOWS 2 TYPES OF ISLETS OF LANGERHANS [J].

BAETENS, D ;

MALAISSELAGAE, F ;

PERRELET, A ;

ORCI, L .

SCIENCE, 1979, 206 (4424) :1323-1325

[3] Nonvertebrate hemoglobins: Structural bases for reactivity [J].

Bolognesi, M ;

Bordo, D ;

Rizzi, M ;

Tarricone, C ;

Ascenzi, P .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1997, 68 (01) :29-68

[4] Triple immunofluorescence staining with antibodies raised in the same species to study the complex innervation pattern of intrapulmonary chemoreceptors [J].

Brouns, I ;

Van Nassauw, L ;

Van Genechten, J ;

Majewski, M ;

Scheuermann, DW ;

Timmermans, JP ;

Adriaensen, D .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2002, 50 (04) :575-582

[5] Nitric oxide moves myoglobin centre stage [J].

Brunori, M .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (04) :209-210

[6] Cytoglobin:: A novel globin type ubiquitously expressed in vertebrate tissues [J].

Burmester, T ;

Ebner, B ;

Weich, B ;

Hankeln, T .

MOLECULAR BIOLOGY AND EVOLUTION, 2002, 19 (04) :416-421

[7] A vertebrate globin expressed in the brain [J].

Burmester, T ;

Weich, B ;

Reinhardt, S ;

Hankeln, T .

NATURE, 2000, 407 (6803) :520-523

[8] A hemoglobin with an optical function [J].

Burr, AHJ ;

Hunt, P ;

Wagar, DR ;

Dewilde, S ;

Blaxter, ML ;

Vanfleteren, JR ;

Moens, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (07) :4810-4815

[9] STUDIES OF THE HEME COORDINATION AND LIGAND-BINDING PROPERTIES OF SOLUBLE GUANYLYL CYCLASE (SGC) - CHARACTERIZATION OF FE(II)SGC AND FE(II)SGC(CO) BY ELECTRONIC ABSORPTION AND MAGNETIC CIRCULAR-DICHROISM SPECTROSCOPIES AND FAILURE OF CO TO ACTIVATE THE ENZYME [J].

BURSTYN, JN ;

YU, AE ;

DIERKS, EA ;

HAWKINS, BK ;

DAWSON, JH .

BIOCHEMISTRY, 1995, 34 (17) :5896-5903

[10] A cooperative oxygen-binding hemoglobin from Mycobacterium tuberculosis [J].

Couture, M ;

Yeh, SR ;

Wittenberg, BA ;

Wittenberg, JB ;

Ouellet, Y ;

Rousseau, DL ;

Guertin, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11223-11228

← 1 2 3 4 5 →