Platelet-derived growth factor induces apoptosis in vascular smooth muscle cells: roles of the Bcl-2 family

被引：38

作者：

Okura, T ^{[1
]}

Igase, M ^{[1
]}

Kitami, Y ^{[1
]}

Fukuoka, T ^{[1
]}

Maguchi, M ^{[1
]}

Kohara, K ^{[1
]}

Hiwada, K ^{[1
]}

机构：

[1] Ehime Univ, Sch Med, Dept Internal Med 2, Matsuyama, Ehime 7910295, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1998年 / 1403卷 / 03期

关键词：

apoptosis; platelet-derived growth factor; vascular smooth muscle cell; Bcl-2; family;

D O I：

10.1016/S0167-4889(98)00065-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis (programmed cell death) is observed in vascular smooth muscle cells (VSMC) in atherosclerotic lesions and stenotic lesions after injury, and modulates the cellularity of these lesions. It is recognized that cell growth and apoptosis are two linked processes. Platelet-derived growth factor (PDGF) induces VSMC proliferation and migration in vitro. We studied the effect of PDGF on apoptosis in VSMC. Cultured rat VSMC were treated with PDGF-AA or PDGF-BB. PDGF-BB induced cell death in cultured VSMC in a time- and dose-dependent manner, but PDGF-AA did not. Gel electrophoresis of genomic DNA and in situ DNA labeling confirmed that the cell death induced by PDGF-BB is apoptosis. PDGF-BB treatment reduced bcl-2 mRNA and bcl-xl mRNA expression, in contrast, induced bcl-xs mRNA expression, linked with the induction of apoptosis in cultured VSMC. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 253

页数：9

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