Burkholderia pseudomallei kills the nematode Caenorhabditis elegans using an endotoxin-mediated paralysis

被引：120

作者：

O'Quinn, AL ^{[1
]}

Wiegand, EM ^{[1
]}

Jeddeloh, JA ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Bacterial Div, Ft Detrick, MD 21702 USA

来源：

CELLULAR MICROBIOLOGY | 2001年 / 3卷 / 06期

关键词：

D O I：

10.1046/j.1462-5822.2001.00118.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated a non-mammalian host model system for fitness in genetic screening for virulence-attenuating mutations in the potential biowarfare agents Burkholderia pseudomallei and Burkholderia mallei. We determined that B. pseudomallei is able to cause 'disease-like' symptoms and kill the nematode Caenorhabditis elegans. Analysis of killing in the surrogate disease model with B. pseudomallei mutants indicated that killing did not require lipopolysaccharide (LPS) O-antigen, aminoglycoside/macrolide efflux pumping, type II pathway-secreted exoenzymes or motility. Burkholderia thailandensis and some strains of Burkholderia cepacia also killed nematodes. Manipulation of the nematode host genotype suggests that the neuromuscular intoxication caused by both B. pseudomallei and B. thailandensis acts in part through a disruption of normal Ca2+ signal transduction. Both species produce a UV-sensitive, gamma-irradiation-resistant, limited diffusion, paralytic agent as part of their nematode pathogenic mechanism. The results of this investigation suggest that killing by B. pseudomallei is an active process in C. elegans, and that the C. elegans model might be useful for the identification of vertebrate animal virulence factors in B. pseudomallei.

引用

页码：381 / 393

页数：13

共 47 条

[31]

MOERMAN DG, 1979, GENETICS, V91, P95

[32] Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei [J].

Moore, RA ;

DeShazer, D ;

Reckseidler, S ;

Weissman, A ;

Woods, DE .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (03) :465-470

[33]

NARITA M, 1982, NATL I ANIM HEALTH Q, V22, P170

[34]

Neubauer H., 1997, Revue Internationale des Services de Sante des Forces Armees, V70, P258

[35]

NIGG CR, 1958, P SCO EXP BIOL MED, V89, P17

[36]

ORMOND RE, 1983, Patent No. 4412991

[37] COMMON VIRULENCE FACTORS FOR BACTERIAL PATHOGENICITY IN PLANTS AND ANIMALS [J].

RAHME, LG ;

STEVENS, EJ ;

WOLFORT, SF ;

SHAO, J ;

TOMPKINS, RG ;

AUSUBEL, FM .

SCIENCE, 1995, 268 (5219) :1899-1902

[38] CaMKII regulates the density of central glutamatergic synapses in vivo [J].

Rongo, C ;

Kaplan, JM .

NATURE, 1999, 402 (6758) :195-199

[39] A CALCIUM-CHANNEL HOMOLOG REQUIRED FOR ADAPTATION TO DOPAMINE AND SEROTONIN IN CAENORHABDITIS-ELEGANS [J].

SCHAFER, WR ;

KENYON, CJ .

NATURE, 1995, 375 (6526) :73-78

[40] Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei [J].

Smith, MD ;

Angus, BJ ;

Wuthiekanun, V ;

White, NJ .

INFECTION AND IMMUNITY, 1997, 65 (10) :4319-4321

← 1 2 3 4 5 →