SHI:: A novel, spherical halovirus isolated from an Australian hypersaline lake

被引:91
作者
Porter, K
Kukkaro, P
Bamford, JKH
Bath, C
Kivelä, HM
Dyall-Smith, ML
Bamford, DH [1 ]
机构
[1] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Dept Biol & Environm Sci, FIN-00014 Helsinki, Finland
[3] Univ Melbourne, Dept Microbiol & Immunol, Melbourne, Vic 3010, Australia
基金
芬兰科学院; 澳大利亚研究理事会;
关键词
salt lake; archaea; archaeal virus; halobacteria; haloarchaea; Haloarcula hispanica;
D O I
10.1016/j.virol.2005.01.043
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel halovirus, SH1, with a spherical morphology is described. Isolated from a hypersaline lake, SH1 is divalent, producing clear plaques on Haloarcula hispanica and a natural Halorubrum isolate. Single-step growth curves gave a latent period of 5-6 h and a burst size of around 200 PFU/cell. The host can differentiate to form tight clusters of thick cell-walled forms, and these were shown to be resistant to infection. Purified virions had no visible tail, were about 70 nm in diameter, and displayed a fragile outer capsid layer, possibly with an underlying membrane component. The structural proteins of the virion were analyzed by SDS-PAGE and several were found to be crosslinked, forming protein complexes. The genome was linear, dsDNA, of approximately 30 kb in length. This morphology and linear genome are features not observed in any other euryarchaeal viruses, but have properties similar to the bacterial virus PRDI. (c) 2005 Published by Elsevier Inc.
引用
收藏
页码:22 / 33
页数:12
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