Layered double hydroxides: An attractive material for electrochemical biosensor design

被引：199

作者：

Shan, D ^{[1
]}

Cosnier, S ^{[1
]}

Mousty, C ^{[1
]}

机构：

[1] Univ Grenoble 1, Inst Chim Mol Grenoble, Lab Electrochim Organ & Photochim Redox, CNRS,UMR 5630, F-38041 Grenoble 9, France

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 15期

关键词：

D O I：

10.1021/ac030030v

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical biosensors for phenol determination were developed based on the immobilization of polyphenol oxidase (PPO) within two different clay matrixes, one anionic (layered double hydroxide, LDH) and the other cationic (Laponite). The biosensor based on the enzyme immobilized in [Zn-Al-Cl] LDH shows greater sensitivity (7807 mA M-1 cm(-2)) and maximum current (492 muA cm(-2)). Biosensor characteristics, such as Michaelis-Menten constant, recycling constant, activation energy, and permeability highlight the advantages of LDH matrixes to immobilize PPO. It appears that LDH provides a favorable environment to PPO activity. The best PPO/[Zn-Al-Cl] configuration was used to determine five different phenol derivatives reaching extremely sensitive detection limits (less than or equal to1 nM).

引用

页码：3872 / 3879

页数：8

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