Determination of estrogens and progestogens by mass spectrometric techniques (GC/MS, LC/MS and LC/MS/MS)

被引:139
作者
Díaz-Cruz, MS
de Alda, MJL
López, R
Barceló, D
机构
[1] CSIC, Inst Invest Quim & Ambientales, Dept Environm Chem, Barcelona 08034, Spain
[2] Univ Barcelona, Dept Analyt Chem, Fac Chem, E-08028 Barcelona, Spain
来源
JOURNAL OF MASS SPECTROMETRY | 2003年 / 38卷 / 09期
关键词
D O I
10.1002/jms.529
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Steroid sex hormones and related synthetic compounds have been shown to provoke alarming estrogenic effects in aquatic organisms, such as feminization, at very low concentrations (ng/L or pg/L). In this work, different chromatographic techniques, namely, gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS), are discussed for the analysis of estrogens, both free and conjugated, and progestogens, and the sensitivities achieved with the various techniques are inter-compared. GC/MS analyses are usually carried out after derivatization of the analytes with bis(trimethylsilyl)trifluoroacetamide (BSTFA). For LC/MS and LC/MS/MS analyses, different instruments, ionization techniques (electrospray (ESI) and atmospheric pressure chemical ionization (APCI)), ionization modes (negative ion (NI) and positive ion (PI)) and monitoring modes (selected ion monitoring (SIM) and selected reaction monitoring (SRM)) are generally employed. Based on sensitivity and selectivity, LC/ESI-MS/MS is generally the method of choice for determination of estrogens in the NI mode and of progestogens in the PI mode (instrumental detection limits (IDLs) 0.1-10 ng/mL). IDLs achieved by LC/ESI-MS in the SIM mode and by LC/ESI-MS/MS in the SRM mode were, in general, comparable, although the selectivity of the latter is significantly higher and essential to avoid false positive determinations in the analysis of real samples. Conclusions and future perspectives are outlined. Copyright (C) 2003 John Wiley Sons, Ltd.
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页码:917 / 923
页数:7
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