Gene expression profiles in pregnant rats treated with T-2 toxin

被引:29
作者
Sehata, S
Kiyosawa, N
Sakuma, K
Ito, K
Yamoto, T
Teranishi, M
Uetsuka, K
Nakayama, H
Doi, K
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Pathol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Sankyo Co Ltd, Med Safety Res Labs, Shizuoka, Japan
关键词
T-2; toxin; pregnant rats; liver; placenta; fetal liver; microarray;
D O I
10.1078/0940-2993-00342
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Pregnant rats on day 13 of gestation were treated orally with T-2 toxin at a single dose of 2 mg/kg and sacrificed at 24 hours after treatment. Histopathologically, apoptosis was increased in the liver. placenta and fetal liver. Microarray analysis was performed to examine the gene expression in the liver, placenta, and fetal liver. The results of microarray analysis showed that the changes in the expression of apoptosis genes. metabolic enzymes and oxidative stress-related genes were detected in these tissues. Suppression of phase I and II enzymes-related genes expression in the liver, and suppression of phase II enzymes-related genes expression in the placenta and fetal liver were observed. Semiquantitive RT-PCR analysis also showed the same results as those of microarray analysis. From the results of microarray analysis and histopathological examination, T-2 toxin seems to induce oxidative stress in these tissues, following the chances in metabolism-related genes expression. These changes may alter the intracellular environments resulting in the induction of apoptosis. Further studies on the gene expression profiles at the earlier time point are necessary to clarify the detailed mechanisms of T-2 toxin-induced toxicity in pregnant rats.
引用
收藏
页码:357 / 366
页数:10
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