Alternative expression of platelet glycoprotein Ib beta mRNA from an adjacent 5' gene with an imperfect polyadenylation signal sequence

被引:55
作者
Zieger, B
Hashimoto, Y
Ware, J
机构
[1] SCRIPPS RES INST, ROON RES CTR ARTERIOSCLEROSIS & THROMBOSIS, LA JOLLA, CA 92037 USA
[2] Scripps Res Inst, DEPT VASC BIOL, LA JOLLA, CA 92037 USA
关键词
gene expression regulation; cloning; molecular; blood platelets; platelet membrane glycoproteins; cell division cycle proteins;
D O I
10.1172/JCI119188
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Glycoprotein (GP) Ib is a major component of the platelet membrane receptor for von Willebrand factor, designated the GP Ib-M-V complex. GP Ib is composed of two subunits (GP Ib alpha and GP Ib beta) each synthesized from separate genes. The 206 amino acid precursor of GP Ib beta is synthesized from a 1.0-kb mRNA expressed by megakaryocytes and was originally characterized from cDNA clones of human erythroleukemic (HEL) cell mRNA, a cell line exhibiting megakaryocytic-like properties. The cell line CHRF-288-11 also exhibits megakaryocytic-like properties, but synthesizes two related GP Ib beta mRNA species of 3.5 and 1.0 kb. We performed cDNA cloning experiments to identify the origin of the 3.5-kb transcript and determine its relationship to the 1.0-kb GP Ib beta mRNA found in megakaryocytes, platelets, and HEL cells. Our cloning experiments demonstrate that the longer transcript results from a nonconsensus polyadenylation recognition sequence, (5')AACAAT(3'), within a separate gene located upstream to the platelet GP Ib beta gene. Zn the absence of normal polyadenylation the more 5' gene uses the polyadenylation site within its 3' neighbor, the platelet GP Ib beta gene. This newly identified 5' gene contains an open reading frame encoding 369 amino acids with a high degree of sequence similarity to an expanding family of GTP-binding proteins.
引用
收藏
页码:520 / 525
页数:6
相关论文
共 30 条