T-DNA insertional mutagenesis for functional genomics in rice

被引：572

作者：

Jeon, JS ^{[1
]}

Lee, S ^{[1
]}

Jung, KH ^{[1
]}

Jun, SH ^{[1
]}

Jeong, DH ^{[1
]}

Lee, J ^{[1
]}

Kim, C ^{[1
]}

Jang, S ^{[1
]}

Lee, S ^{[1
]}

Yang, K ^{[1
]}

Nam, J ^{[1
]}

An, K ^{[1
]}

Han, MJ ^{[1
]}

Sung, RJ ^{[1
]}

Choi, HS ^{[1
]}

Yu, JH ^{[1
]}

Choi, JH ^{[1
]}

Cho, SY ^{[1
]}

Cha, SS ^{[1
]}

Kim, SI ^{[1
]}

An, G ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Natl Res Lab Plant Funct Genom, Pohang 790784, South Korea

来源：

PLANT JOURNAL | 2000年 / 22卷 / 06期

关键词：

D O I：

10.1046/j.1365-313x.2000.00767.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can be estimated that approximately 25 700 taggings have been generated. The binary vector used in the insertion contained the promoterless beta-glucuronidase (GUS) reporter gene with an intron and multiple splicing donors and acceptors immediately next to the right border. Therefore, this gene trap vector is able to detect a gene fusion between GUS and an endogenous gene, which is tagged by T-DNA. Histochemical GUS assays were carried out in the leaves and roots from 5353 lines, mature flowers from 7026 lines, and developing seeds from 1948 lines. The data revealed that 1.6-2.1% of tested organs were GUS-positive in the tested organs, and that their GUS expression patterns were organ- or tissue-specific or ubiquitous in all parts of the plant. The large population of T-DNA-tagged lines will be useful for identifying insertional mutants in various genes and for discovering new genes in rice.

引用

页码：561 / 570

页数：10

共 35 条

[1]

Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P211, DOI DOI 10.1007/BF02672069

[2] T-DNA insertion mutagenesis in Arabidopsis: Going back and forth [J].