p14ARF interacts with N-Myc and inhibits its transcriptional activity

被引:14
作者
Amente, Stefano
Gargano, Barbara
Diolaiti, Daniel
Della Valle, Giuliano
Lania, Luigi
Majello, Barbara [1 ]
机构
[1] Univ Naples Federico II, Dept Struct & Funct Biol, Naples, Italy
[2] Univ Bologna, Dept Biol, I-40126 Bologna, Italy
关键词
p14(ARF); N-Myc; C-Myc; protein interactions; transcription; tumor suppressor;
D O I
10.1016/j.febslet.2007.01.062
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we report that the human p14(ARF) associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14(ARF) are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14(ARF) protein is involved in binding to c-Mye and N-Myc proteins. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:821 / 825
页数:5
相关论文
共 25 条
[1]   Transcriptional regulation and transformation by MYC proteins [J].
Adhikary, S ;
Eilers, M .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (08) :635-645
[2]   p14ARF directly interacts with Myc through the Myc BoxII domain [J].
Amente, Stefano ;
Gargano, Barbara ;
Varrone, Francesca ;
Ruggiero, Luca ;
Dominguez-Sola, David ;
Lania, Luigi ;
Majello, Barbara .
CANCER BIOLOGY & THERAPY, 2006, 5 (03) :287-291
[3]   Induction of apoptosis by p75 neurotrophin receptor in human neuroblastoma cells [J].
Bunone, G ;
Mariotti, A ;
Compagni, A ;
Morandi, E ;
DellaValle, G .
ONCOGENE, 1997, 14 (12) :1463-1470
[4]   EMBRYONIC LETHALITY IN MICE HOMOZYGOUS FOR A TARGETED DISRUPTION OF THE N-MYC GENE [J].
CHARRON, J ;
MALYNN, BA ;
FISHER, P ;
STEWART, V ;
JEANNOTTE, L ;
GOFF, SP ;
ROBERTSON, EJ ;
ALT, FW .
GENES & DEVELOPMENT, 1992, 6 (12A) :2248-2257
[5]  
Cole MD, 2006, CURR TOP MICROBIOL, V302, P33
[6]   New and unexpected: forkhead meets ARF [J].
Costa, RH ;
Kalinichenko, VV ;
Major, ML ;
Raychaudhuri, P .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (01) :42-48
[7]   Mechanism of transcriptional activation by the Myc oncoproteins [J].
Cowling, Victoria H. ;
Cole, Michael D. .
SEMINARS IN CANCER BIOLOGY, 2006, 16 (04) :242-252
[8]   A conserved Myc protein domain, MBIV, regulates DNA binding, apoptosis, transformation, an G2 arrest [J].
Cowling, Victoria H. ;
Chandriani, Sanjay ;
Whitfield, Michael L. ;
Cole, Michael D. .
MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (11) :4226-4239
[9]   ARF directly binds DP1:: Interaction with DP1 coincides with the G1 arrest function of ARF [J].
Datta, A ;
Sen, J ;
Hagen, J ;
Korgaonkar, CK ;
Caffrey, M ;
Quelle, DE ;
Hughes, DE ;
Ackerson, TJ ;
Costa, RH ;
Raychaudhuri, P .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (18) :8024-8036
[10]   Myc-ARF (alternate reading frame) interaction inhibits the functions of Myc [J].
Datta, A ;
Nag, A ;
Pan, W ;
Hay, N ;
Gartel, AL ;
Colamonici, O ;
Mori, Y ;
Raychaudhuri, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (35) :36698-36707