Intersegmental interactions in supercoiled DNA: atomic force microscope study

被引:42
作者
Shlyakhtenko, LS
Miloseska, L
Potaman, VN
Sinden, RR
Lyubchenko, YL [1 ]
机构
[1] Arizona State Univ, Dept Microbiol, Tempe, AZ 85287 USA
[2] Arizona State Univ, Dept Biol, Tempe, AZ 85287 USA
[3] Texas A&M Univ, Syst Hlth Sci Ctr, Ctr Genome Res, Inst Biosci & Technol, Houston, TX USA
关键词
AFM; DNA supercoiling; surface functionalization; silanes;
D O I
10.1016/S0304-3991(03)00051-2
中图分类号
TH742 [显微镜];
学科分类号
摘要
Intersegmental interactions in DNA facilitated by the neutralization of electrostatic repulsion was studied as a function of salt concentration and DNA supercoiling. DNA samples with defined superhelical densities were deposited onto aminopropyl mica at different ionic conditions and imaged in air after drying of the samples. Similar to hydrodynamic data, we did not observe a collapse of supercoiled DNA, as proposed earlier by cryo-EM studies. Instead, the formation of the contacts between DNA helices within supercoiled loops with no visible space between the duplexes was observed. The length of such close contacts increased upon increasing NaCl concentration. DNA supercoiling was a critical factor for the stabilization of intersegmental contacts. Implications of the observed effect for understanding DNA compaction in the cell and for regulation DNA transactions via interaction of distantly separated DNA regions are discussed. (C) 2003 Published by Elsevier Science B.V.
引用
收藏
页码:263 / 270
页数:8
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