Capillary electrophoresis of arsenic compounds with indirect fluorescence detection

被引:31
作者
Huang, YM [1 ]
Whang, CW [1 ]
机构
[1] Tunghai Univ, Dept Chem, Taichung 407, Taiwan
关键词
capillary electrophoresis; arsenic compounds; indirect fluorescence detection; fluorescein;
D O I
10.1002/elps.1150191217
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A capillary electrophoresis (CE)-indirect fluorescence detection method for arsenic compounds is described. The five arsenic species, viz., arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) and phenylarsonate (PhA), were efficiently separated by CE in 8 min with an 1.5 mM fluorescein solution at pH 9.8. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent arsenic species. Linearity (r greater than or equal to 0.996) of more than two orders of magnitude was generally obtained. The relative standard deviation (RSD) values were in the ranges 0.4-0.7% and 2.2-8.2% for migration times and peak areas, respectively. The concentration limits of detection (CLODs) for the arsenic compounds studied were between 0.04 and 0.16 mu g/mL (as arsenic). The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each arsenic species. The applicability of the method for the analysis of ground water was examined.
引用
收藏
页码:2140 / 2144
页数:5
相关论文
共 26 条
[1]   ANALYSIS OF ORGANIC AND NONORGANIC ARSENIOUS OR SELENIOUS COMPOUNDS BY CAPILLARY ELECTROPHORESIS [J].
ALBERT, M ;
DEMESMAY, C ;
ROCCA, JL .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1995, 351 (4-5) :426-432
[2]  
ALBERT M, 1992, ANALUSIS, V20, P383
[3]   INDIRECT FLUORESCENCE DETECTION IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY [J].
AMANKWA, LN ;
KUHR, WG .
ANALYTICAL CHEMISTRY, 1991, 63 (17) :1733-1737
[4]   SEPARATION OF DNA RESTRICTION FRAGMENTS USING CAPILLARY ELECTROPHORESIS [J].
CHAN, KC ;
WHANG, CW ;
YEUNG, ES .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (9-10) :1941-1962
[5]   CAPILLARY ZONE ELECTROPHORESIS OF 11 PRIORITY PHENOLS WITH INDIRECT FLUORESCENCE DETECTION [J].
CHAO, YC ;
WHANG, CW .
JOURNAL OF CHROMATOGRAPHY A, 1994, 663 (02) :229-237
[6]   UTILIZATION OF FLUORESCEIN SODIUM-SALT IN LASER-INDUCED INDIRECT FLUOROMETRIC DETECTION OF IONS SEPARATED BY CAPILLARY ZONE ELECTROPHORESIS [J].
DESBENE, PL ;
MORIN, CJ ;
MONVERNAY, AMD ;
GROULT, RS .
JOURNAL OF CHROMATOGRAPHY A, 1995, 689 (01) :135-148
[7]   COUPLED CHROMATOGRAPHY-ATOMIC SPECTROMETRY FOR ARSENIC SPECIATION - A COMPARATIVE-STUDY [J].
EBDON, L ;
HILL, S ;
WALTON, AP ;
WARD, RW .
ANALYST, 1988, 113 (08) :1159-1165
[8]  
GJERDE DT, 1991, J LIQ CHROMATOGR, V47, P213
[9]   INDIRECT FLUORIMETRIC DETECTION AND QUANTIFICATION IN CAPILLARY ZONE ELECTROPHORESIS OF INORGANIC ANIONS AND NUCLEOTIDES [J].
GROSS, L ;
YEUNG, ES .
JOURNAL OF CHROMATOGRAPHY, 1989, 480 :169-178
[10]   INDIRECT FLUOROMETRIC DETECTION OF CATIONS IN CAPILLARY ZONE ELECTROPHORESIS [J].
GROSS, L ;
YEUNG, ES .
ANALYTICAL CHEMISTRY, 1990, 62 (05) :427-431