Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assays for Rapid Diagnosis of Pandemic Influenza A/H1N1 2009 Virus

被引:35
作者
Nakauchi, Mina [1 ]
Yoshikawa, Tetsushi [2 ]
Nakai, Hidetaka [2 ]
Sugata, Ken [2 ]
Yoshikawa, Akiko [2 ]
Asano, Yoshizo [2 ]
Ihira, Masaru [3 ]
Tashiro, Masato [1 ]
Kageyama, Tsutomu [1 ]
机构
[1] Natl Inst Infect Dis, Influenza Virus Res Ctr, Tokyo 2080011, Japan
[2] Fujita Hlth Univ, Sch Med, Dept Pediat, Aichi, Japan
[3] Fujita Hlth Univ, Sch Hlth Sci, Fac Clin Engn, Aichi, Japan
关键词
rapid diagnosis; RT-LAMP; pandemic influenza A/H1N1 2009 virus; A H1N1 VIRUS; INFECTION; PCR; MILWAUKEE; WISCONSIN;
D O I
10.1002/jmv.21934
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two genetic diagnosis systems using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology were evaluated: one for detecting the HA gene of the pandemic influenza A/H1N1 2009 virus (H1pdm RT-LAMP) and the other for detecting the matrix gene of the influenza A virus (TypeA RT-LAMP). The competence of these two RT-LAMP assay kits for the diagnosis of the pandemic influenza A/H1N1 2009 virus was compared using real-time RT-PCR assays developed recently on viruses isolated and clinical specimens collected from patients with suspected infection. TypeA RT-LAMP and H1pdm RT-LAMP showed almost the same sensitivity as real-time RT-PCR for viruses isolated. The sensitivity and specificity of TypeA RT-LAMP and H1pdm AT-LAMP were 96.3% and 88.9%, respectively, for clinical specimens. Considering that the ability of the two RT-LAMP assay kits for detection of the pandemic influenza A/H1N1 2009 virus was comparable to that of the real-time RT-PCR assays, and that the assays were completed within 1 hr and did not require any expensive equipment, these two RT-LAMP assays are promising rapid diagnostic tests for the pandemic influenza A/H1N1 2009 virus at the hospital bedside. J. Med. Virol. 83:10-15, 2011. (C) 2010 Wiley-Liss, Inc.
引用
收藏
页码:10 / 15
页数:6
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