Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus

被引：39

作者：

Anavi-Goffer, S

McKay, NG

Ashford, MLJ

Coutts, AA ^{[1
]}

机构：

[1] Univ Aberdeen, Inst Med Sci, Dept Biomed Sci, Aberdeen AB25 2ZD, Scotland

[2] Univ Dundee, Dept Pharmacol & Neurosci, Inst Neurosci, Dundee DD1 9SY, Scotland

来源：

NEUROSCIENCE LETTERS | 2002年 / 319卷 / 01期

基金：

英国惠康基金;

关键词：

sensory neurones; calbindin; ileum; colon; rat; reverse transcription-polymerase chain reaction;

D O I：

10.1016/S0304-3940(01)02480-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：53 / 57

页数：5

共 21 条

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