Regulators acting in combinatorial codes also act independently in single differentiating neurons

被引:69
作者
Allan, DW
Park, D
St Pierre, SE
Taghert, PH
Thor, S
机构
[1] Harvard Univ, Sch Med, Dept Neurobiol, Boston, MA 02115 USA
[2] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA
关键词
D O I
10.1016/j.neuron.2005.01.026
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the Drosophila ventral nerve cord, a small number of neurons express the LIM-homeodomain gene apterous (ap). These ap neurons can be subdivided based upon axon pathfinding and their expression of neuropeptidergic markers. ap, the zinc finger gene squeeze, the bHLH gene dimmed, and the BMP pathway are all required for proper specification of these cells. Here, using several ap neuron terminal differentiation markers, we have resolved how each of these factors contributes to ap neuron diversity. We find that these factors interact genetically and biochemically in subtype-specific combinatorial codes to determine certain defining aspects of ap neuron subtype identity. However, we also find that ap, dimmed, and squeeze additionally act independently of one another to specify certain other defining aspects of ap neuron subtype identity. Therefore, within single neurons, we show that single regulators acting in numerous molecular contexts differentially specify multiple subtype-specific traits.
引用
收藏
页码:689 / 700
页数:12
相关论文
共 46 条
[1]   Alteration of cell fate by ectopic expression of Drosophila glial cells missing in non-neural cells [J].
Akiyama-Oda, Y ;
Hosoya, T ;
Hotta, Y .
DEVELOPMENT GENES AND EVOLUTION, 1998, 208 (10) :578-585
[2]   Specification of neuropeptide cell identity by the integration of retrograde BMP signaling and a combinatorial transcription factor code [J].
Allan, DW ;
St Pierre, SE ;
Miguel-Aliaga, I ;
Thor, S .
CELL, 2003, 113 (01) :73-86
[3]  
Benveniste RJ, 1998, DEVELOPMENT, V125, P4757
[4]   Proneural genes and the specification of neural cell types [J].
Bertrand, N ;
Castro, DS ;
Guillemot, F .
NATURE REVIEWS NEUROSCIENCE, 2002, 3 (07) :517-530
[5]   2ND-SITE MODIFIERS OF THE SPLIT MUTATION OF NOTCH DEFINE GENES INVOLVED IN NEUROGENESIS IN DROSOPHILA-MELANOGASTER [J].
BRAND, M ;
CAMPOSORTEGA, JA .
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY, 1990, 198 (05) :275-285
[6]   Phox2 genes -: from patterning to connectivity [J].
Brunet, JF ;
Pattyn, A .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2002, 12 (04) :435-440
[7]   Tracing axons [J].
Callahan, CA ;
Yoshikawa, S ;
Thomas, JB .
CURRENT OPINION IN NEUROBIOLOGY, 1998, 8 (05) :582-586
[8]  
Castanon I, 2001, DEVELOPMENT, V128, P3145
[9]   ORGANIZATION AND EXPRESSION OF THE DROSOPHILA FMRFAMIDE-RELATED PROHORMONE GENE [J].
CHIN, AC ;
REYNOLDS, ER ;
SCHELLER, RH .
DNA AND CELL BIOLOGY, 1990, 9 (04) :263-271
[10]   Delta is a ventral to dorsal signal complementary to Serrate, another Notch ligand in Drosophila wing formation [J].
Doherty, D ;
Feger, G ;
YoungerShepherd, S ;
Jan, LY ;
Jan, YN .
GENES & DEVELOPMENT, 1996, 10 (04) :421-434