The human solute carrier SLC41A1 belongs to a novel eukaryotic subfamily with homology to prokaryotic MgtE Mg2+ transporters

被引：76

作者：

Wabakken, T ^{[1
]}

Rian, E ^{[1
]}

Kveine, M ^{[1
]}

Aasheim, HC ^{[1
]}

机构：

[1] Norwegian Radium Hosp, Dept Immunol, N-0310 Oslo, Norway

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 03期

关键词：

eukaryotic gene family; prokaryotic MgtE;

D O I：

10.1016/S0006-291X(03)01030-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the first identification and structural characterization of a eukaryotic protein with homology to the bacterial MgtE family of potential Mg2+ transporters. This human protein, denoted solute carrier family 41 member 1 (SLC41A1), consists of 5 13 amino acids with an estimated molecular weight of 56 kDa. Computer analysis of the protein structure reveals that the protein consists of 10 putative transmembrane domains and includes two distinct domains highly. homologous to the integral membrane part of the bacterial MgtE protein family. The gene encoding SLC41A1 is found on chromosome 1 (1q31-32) and the protein coding sequence is found on 10 exons. A 5-kb long transcript is identified in various human tissues with highest expression levels in heart and testis. We have also identified 10 SLC41A1 homologs in Homo sapiens, Mus musculus, Drosophila melanogaster, Anopheles gambiae, and Caenorhabditis elegans, and propose that these hypothetical proteins belong to a novel eukaryotic gene family. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：718 / 724

页数：7

共 25 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[3] Differential localization and operation of distinct Mg2+ transporters in apical and basolateral sides of rat liver plasma membrane [J].

Cefaratti, C ;

Romani, A ;

Scarpa, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (06) :3772-3780

[4] IDENTIFICATION OF CALCINEURIN AS A KEY SIGNALING ENZYME IN LYMPHOCYTE-T ACTIVATION [J].

CLIPSTONE, NA ;

CRABTREE, GR .

NATURE, 1992, 357 (6380) :695-697

[5] ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT [J].

EVAN, GI ;

LEWIS, GK ;

RAMSAY, G ;

BISHOP, JM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3610-3616

[6]

Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X

[7]

GUNTHER T, 1986, MAGNESIUM, V5, P53

[8]

Kehres D G, 1998, Microb Comp Genomics, V3, P151, DOI 10.1089/omi.1.1998.3.151

[9] Initiation of translation in prokaryotes and eukaryotes [J].

Kozak, M .

GENE, 1999, 234 (02) :187-208

[10] The protein kinase complement of the human genome [J].

Manning, G ;

Whyte, DB ;

Martinez, R ;

Hunter, T ;

Sudarsanam, S .

SCIENCE, 2002, 298 (5600) :1912-+

← 1 2 3 →