The human solute carrier SLC41A1 belongs to a novel eukaryotic subfamily with homology to prokaryotic MgtE Mg2+ transporters

被引：76

作者：

Wabakken, T ^{[1
]}

Rian, E ^{[1
]}

Kveine, M ^{[1
]}

Aasheim, HC ^{[1
]}

机构：

[1] Norwegian Radium Hosp, Dept Immunol, N-0310 Oslo, Norway

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 03期

关键词：

eukaryotic gene family; prokaryotic MgtE;

D O I：

10.1016/S0006-291X(03)01030-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the first identification and structural characterization of a eukaryotic protein with homology to the bacterial MgtE family of potential Mg2+ transporters. This human protein, denoted solute carrier family 41 member 1 (SLC41A1), consists of 5 13 amino acids with an estimated molecular weight of 56 kDa. Computer analysis of the protein structure reveals that the protein consists of 10 putative transmembrane domains and includes two distinct domains highly. homologous to the integral membrane part of the bacterial MgtE protein family. The gene encoding SLC41A1 is found on chromosome 1 (1q31-32) and the protein coding sequence is found on 10 exons. A 5-kb long transcript is identified in various human tissues with highest expression levels in heart and testis. We have also identified 10 SLC41A1 homologs in Homo sapiens, Mus musculus, Drosophila melanogaster, Anopheles gambiae, and Caenorhabditis elegans, and propose that these hypothetical proteins belong to a novel eukaryotic gene family. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：718 / 724

页数：7

共 25 条

[11] Magnesium transport in prokaryotes [J].

Moncrief, MBC ;

Maguire, ME .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 1999, 4 (05) :523-527

[12]

OKABE M, 1987, BLOOD, V69, P990

[13] A signal sequence trap based on cell enrichment using Anti-CD19 antibody coated magnetic beads [J].

Rian, E ;

Wabakken, T ;

Hauge, H ;

Aasheim, HC .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2001, 54 (03) :280-284

[14]

ROMANI A, 1993, MINER ELECTROL METAB, V19, P282

[15] REGULATION OF CELL MAGNESIUM [J].

ROMANI, A ;

SCARPA, A .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 298 (01) :1-12

[16]

Sambrook J., 1989, Molecular Cloning: a Laboratory Manual, V2nd

[17] Classification of introns: U2-type or U12-type [J].

Sharp, PA ;

Burge, CB .

CELL, 1997, 91 (07) :875-879

[18] Microbial magnesium transport: unusual transporters searching for identity [J].

Smith, RL ;

Maguire, ME .

MOLECULAR MICROBIOLOGY, 1998, 28 (02) :217-226

[19] CLONING AND CHARACTERIZATION OF MGTE, A PUTATIVE NEW CLASS OF MG2+ TRANSPORTER FROM BACILLUS-FIRMUS OF4 [J].

SMITH, RL ;

THOMPSON, LJ ;

MAGUIRE, ME .

JOURNAL OF BACTERIOLOGY, 1995, 177 (05) :1233-1238

[20]

Sonnhammer E L, 1998, Proc Int Conf Intell Syst Mol Biol, V6, P175

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