Diagnostic utility of tenascin-C for evaluation of the activity of human acute myocarditis

被引:64
作者
Morimoto, S
Imanaka-Yoshida, K
Hiramitsu, S
Kato, S
Ohtsuki, M
Uemura, A
Kato, Y
Nishikawa, T
Toyozaki, T
Hishida, H
Yoshida, T
Hiroe, M
机构
[1] Mie Univ, Sch Med, Dept Pathol, Tsu, Mie 5148507, Japan
[2] Fujita Hlth Univ, Sch Med, Dept Internal Med, Div Cardiol, Toyoake, Aichi 47011, Japan
[3] Tokyo Womens Med Univ, Dept Pathol, Tokyo, Japan
[4] Chiba Univ, Sch Med, Inst Pulm Canc Res, Div Pathol, Chiba 280, Japan
[5] Int Med Ctr Japan, Dept Nephrol & Cardiol, Tokyo, Japan
关键词
tenascin; extracellular matrix; myocarditis; heart; inflammation; human; biopsy; diagnosis; immunostaining; in situ hybridization;
D O I
10.1002/path.1730
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Tenascin-C (TN-C) is an extracellular matrix protein that is expressed transiently in close association with tissue remodelling in various body sites. In the heart, TN-C is only present during early stages of development, is not expressed in the normal adult, but reappears in pathological states. The purpose of this study was to analyse the expression of TN-C in myocardial tissue from myocarditis patients, and to evaluate the diagnostic value of immunostaining for TN-C in the assessment of inflammatory activity in biopsy specimens. A total of 113 biopsy specimens obtained from 32 patients with a clinical diagnosis of acute myocarditis were examined by immunohistochemistry and in situ hybridization for TN-C. The immunostaining was semi-quantified and compared with histological diagnosis according to the Dallas criteria. Furthermore, serial biopsies from 22 patients were taken during convalescence, and sequential changes in TN-C levels were analysed. Expression of TN-C was specifically detected in endomyocardial biopsy specimens from patients with active-stage inflammation, and disappeared in healed stages. The degree of expression of TNC correlated with the severity of histological lesions. These data suggest that TN-C reflects disease activity in cases of human myocarditis. Immunostaining for TN-C could enhance the sensitivity and accuracy of diagnosis using biopsy specimens. Copyright (C) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
引用
收藏
页码:460 / 467
页数:8
相关论文
共 40 条
[1]   Active versus borderline myocarditis: clinicopathological correlates and prognostic implications [J].
Angelini, A ;
Crosato, M ;
Boffa, GM ;
Calabrese, F ;
Calzolari, V ;
Chioin, R ;
Daliento, L ;
Thiene, G .
HEART, 2002, 87 (03) :210-215
[2]  
Aretz H T, 1987, Am J Cardiovasc Pathol, V1, P3
[3]   Cell-mediated cytotoxicity in hearts with dilated cardiomyopathy: Correlation with interstitial fibrosis and foci of activated T lymphocytes [J].
Badorff, C ;
Noutsias, M ;
Kuhl, U ;
Schultheiss, HP .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1997, 29 (02) :429-434
[4]   Tenascins: regulation and putative functions during pathological stress [J].
Chiquet-Ehrismann, R ;
Chiquet, M .
JOURNAL OF PATHOLOGY, 2003, 200 (04) :488-499
[5]  
DAVIES MJ, 1992, BRIT HEART J, V68, P346
[6]   ACTIVE MYOCARDITIS IN THE SPECTRUM OF ACUTE DILATED CARDIOMYOPATHIES - CLINICAL-FEATURES, HISTOLOGIC CORRELATES, AND CLINICAL OUTCOME [J].
DEC, GW ;
PALACIOS, IF ;
FALLON, JT ;
ARETZ, HT ;
MILLS, J ;
LEE, DCS ;
JOHNSON, RA .
NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (14) :885-890
[7]   Detection of myocardial infarction by immunohistological staining for C9 on formalin fixed, paraffin wax embedded sections [J].
Doran, JP ;
Howie, AJ ;
Townend, JN ;
Bonser, RS .
JOURNAL OF CLINICAL PATHOLOGY, 1996, 49 (01) :34-37
[8]   DIAGNOSIS AND CLASSIFICATION OF MYOCARDITIS BY ENDOMYOCARDIAL BIOPSY [J].
FENOGLIO, JJ ;
URSELL, PC ;
KELLOGG, CF ;
DRUSIN, RE ;
WEISS, MB .
NEW ENGLAND JOURNAL OF MEDICINE, 1983, 308 (01) :12-18
[9]   Active interstitial remodeling: An important process in the hibernating human myocardium [J].
Frangogiannis, NG ;
Shimoni, S ;
Chang, SM ;
Ren, GF ;
Dewald, O ;
Gersch, C ;
Shan, K ;
Aggeli, C ;
Reardon, M ;
Letsou, GV ;
Espada, R ;
Ramchandani, M ;
Entman, ML ;
Zoghbi, WA .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2002, 39 (09) :1468-1474
[10]   LONG-TERM OUTCOME OF PATIENTS WITH BIOPSY-PROVED MYOCARDITIS - COMPARISON WITH IDIOPATHIC DILATED CARDIOMYOPATHY [J].
GROGAN, M ;
REDFIELD, MM ;
BAILEY, KR ;
REEDER, GS ;
GERSH, BJ ;
EDWARDS, WD ;
RODEHEFFER, RJ .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1995, 26 (01) :80-84