A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens

被引：29

作者：

Sakabe, Saori ^{[1
,7
]}

Sakoda, Yoshihiro ^{[1
]}

Haraguchi, Yoshinari ^{[1
]}

Isoda, Norikazu ^{[1
]}

Soda, Kosuke ^{[1
]}

Takakuwa, Hiroki ^{[1
,2
]}

Saijo, Kazue ^{[3
]}

Sawata, Akira ^{[3
]}

Kume, Katsumi ^{[3
]}

Hagiwara, Junko ^{[4
]}

Tuchiya, Kotaro ^{[4
]}

Lin, Zhifeng ^{[4
]}

Sakamoto, Ryuichi ^{[5
]}

Imamura, Takashi ^{[5
]}

Sasaki, Takashi ^{[6
]}

Kokumai, Norihide ^{[6
]}

Kawaoka, Yoshihiro ^{[7
]}

Kida, Hiroshi ^{[1
,8
]}

机构：

[1] Hokkaido Univ, Microbiol Lab, Dept Dis Control, Grad Sch Vet Med, Sapporo, Hokkaido 0600818, Japan

[2] Kyoto Sangyo Univ, Avian Influenza Res Ctr, Kyoto 6038555, Japan

[3] Kitasato Inst, Biol Res Ctr, Kitamoto, Saitama 3640026, Japan

[4] Nippon Inst Biol Sci, Res Dept, Tokyo 1980024, Japan

[5] Chemo Sero Therapeut Res Inst, Div 2, Res Dept 2, Kumamoto 8691298, Japan

[6] Kyoto Biken Labs Inc, Avian Biol Dept, Kyoto 6110041, Japan

[7] Univ Tokyo, Div Virol, Dept Microbiol & Immunol, Inst Med Sci, Tokyo 1088639, Japan

[8] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido 0010020, Japan

来源：

VACCINE | 2008年 / 26卷 / 17期

关键词：

avian influenza virus; H7; subtype; migratory ducks; vaccine;

D O I：

10.1016/j.vaccine.2008.02.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

During 2001-2004, 41 H7 influenza viruses (2 H7N1 and 39 H7N7 strains) were isolated from fecal samples of migratory ducks that flew from Siberia in the autumn of each year to Japan and Mongolia. A phylogenetic analysis of the hemagglutinin (HA) genes of the nine representative isolates revealed that they belonged to the Eurasian Lineage and the deduced amino acid sequence at the cleavage site of the HAs represented apathogenic profiles. One of the H7 isolates A/duck/Mongolia/736/02 (H7N7) was chosen from these H7 isolates for the preparation of the test vaccine. To improve the growth potential of A/duck/Mongolia/ 736/02 (H7N7) in chicken embryos, A/duck/Hokkaido/Vac-2/04 (H7N7) was generated by genetic reassortment between A/duck/Mongolia/736/02 (H7N7) as the donor of the PB2, PB1, PA, HA, NA, and NS genes and A/duck/Hokkaido/49/98 (H9N2) as that of NP and M genes. The test vaccine was prepared as follows; A/duck/Hokkaido/Vac-2/04 (H7N7) was propagated in chicken embryos and the virus in the allantoic fluid was inactivated and adjuvanted to form an oil-in-water emulsion. The test vaccine conferred immunity to chickens, completely protecting the manifestation of clinical signs against the challenge with lethal dose of H7 highly pathogenic avian influenza virus. These results indicate that influenza viruses isolated from natural reservoirs are useful for vaccine strains. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2127 / 2134

页数：8

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