Characterization and modeling of monolithic stationary phases: application to preparative chromatography

被引:39
作者
Ghose, S [1 ]
Cramer, SM [1 ]
机构
[1] Rensselaer Polytech Inst, Dept Chem Engn, Troy, NY 12180 USA
基金
美国国家科学基金会;
关键词
monolithic stationary phases; steric mass action; mass transport models; stationary phases; LC; ion exchange;
D O I
10.1016/S0021-9673(01)01115-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A methodology for characterizing and modeling preparative separations on monolithic ion-exchange stationary phases is presented. A dimensionless group analysis was carried out to determine the relative importance of mass transfer and kinetic resistances on this stationary phase. In contrast to conventional beaded morphologies, the continuous bed stationary phase was found to possess enhanced mass transport properties resulting in kinetic resistance as the dominant non-ideality. Accordingly, a reaction-dispersive steric-mass action formalism was successfully utilized for simulating preparative displacement chromatography on this resin. Since kinetics were found to be important on this column morphology, mobile phase salt concentration was found to be an important variable during displacement chromatography on this stationary phase. An increase in the mobile phase salt concentration was found to significantly improve the displacement separation of a model protein mixture. The formalism presented in this paper provides a better understanding of preparative chromatography in monolithic resin systems and a means of simulating separations on this class of chromatographic stationary phases. (C) 2001 Elsevier Science B.V All rights reserved.
引用
收藏
页码:13 / 23
页数:11
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