Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atx1 mutants

被引：109

作者：

Alvarez-Venegas, R ^{[1
]}

Avramova, Z ^{[1
]}

机构：

[1] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA

来源：

NUCLEIC ACIDS RESEARCH | 2005年 / 33卷 / 16期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/gki830

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Covalent modifications of histone-tail amino acid residues communicate information via a specific 'histone code'. Here, we report histone H3-tail lysine methylation profiles of several Arabidopsis genes in correlation with their transcriptional activity and the input of the epigenetic factor ARABIDOPSIS HOMOLOG OF TRITHORAX (ATX1) at ATX1-regulated loci. By chromatin immunoprecipitation (ChIP) assays, we compared modification patterns of a constitutively expressed housekeeping gene, of a tissue-specific gene, and among genes that differed in degrees of transcriptional activity. Our results suggest that the di-methylated isoform of histone H3-lysine4 (m(2)K4/H3) provide a general mark for gene-related sequences distinguishing them from non-transcribed regions. Lys-4 (K4/H3), lys-9 (K9/H3) and lys-27 (K27/H3) nucleosome methylation patterns of plant genes may be gene-, tissue- or development-regulated. Absence of nucleosomes from the LTP-promotor was not sufficient to provoke robust transcription in mutant atx1-leaf chromatin, suggesting that the mechanism repositioning nucleosomes at transition to flowering functioned independently of ATX1.

引用

页码：5199 / 5207

页数：9

共 30 条

[1] SET-domain proteins of the Su(var)3-9, E(z) and trithorax families [J].

Alvarez-Venegas, R ;

Avramova, Z .

GENE, 2002, 285 (1-2) :25-37

[2] ATX-1, an Arabidopsis homolog of trithorax, activates flower homeotic genes [J].

Alvarez-Venegas, R ;

Pien, S ;

Sadder, M ;

Witmer, X ;

Grossniklaus, U ;

Avramova, Z .

CURRENT BIOLOGY, 2003, 13 (08) :627-637

[3] Heterochromatin in animals and plants. Similarities and differences [J].

Avramova, ZV .

PLANT PHYSIOLOGY, 2002, 129 (01) :40-49

[4] Vernalization requires epigenetic silencing of FLC by histone methylation [J].

Bastow, R ;

Mylne, JS ;

Lister, C ;

Lippman, Z ;

Martienssen, RA ;

Dean, C .

NATURE, 2004, 427 (6970) :164-167

[5] The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes [J].

Baumbusch, LO ;

Thorstensen, T ;

Krauss, V ;

Fischer, A ;

Naumann, K ;

Assalkhou, R ;

Schulz, I ;

Reuter, G ;

Aalen, RB .

NUCLEIC ACIDS RESEARCH, 2001, 29 (21) :4319-4333

[6] Methylation of histone H3 Lys 4 in coding regions of active genes [J].

Bernstein, BE ;

Humphrey, EL ;

Erlich, RL ;

Schneider, R ;

Bouman, P ;

Liu, JS ;

Kouzarides, T ;

Schreiber, SL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (13) :8695-8700

[7]

García-Olmedo F, 1998, BIOPOLYMERS, V47, P479, DOI 10.1002/(SICI)1097-0282(1998)47:6<479::AID-BIP6>3.0.CO

[8]

2-K

[9] Profiling histone modification patterns in plants using genomic tiling microarrays [J].

Gendrel, AV ;

Lippman, Z ;

Martienssen, R ;

Colot, V .

NATURE METHODS, 2005, 2 (03) :213-218

[10]

Henikoff S, 1998, GENETICS, V149, P307

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