Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atx1 mutants

被引：109

作者：

Alvarez-Venegas, R ^{[1
]}

Avramova, Z ^{[1
]}

机构：

[1] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA

来源：

NUCLEIC ACIDS RESEARCH | 2005年 / 33卷 / 16期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/gki830

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Covalent modifications of histone-tail amino acid residues communicate information via a specific 'histone code'. Here, we report histone H3-tail lysine methylation profiles of several Arabidopsis genes in correlation with their transcriptional activity and the input of the epigenetic factor ARABIDOPSIS HOMOLOG OF TRITHORAX (ATX1) at ATX1-regulated loci. By chromatin immunoprecipitation (ChIP) assays, we compared modification patterns of a constitutively expressed housekeeping gene, of a tissue-specific gene, and among genes that differed in degrees of transcriptional activity. Our results suggest that the di-methylated isoform of histone H3-lysine4 (m(2)K4/H3) provide a general mark for gene-related sequences distinguishing them from non-transcribed regions. Lys-4 (K4/H3), lys-9 (K9/H3) and lys-27 (K27/H3) nucleosome methylation patterns of plant genes may be gene-, tissue- or development-regulated. Absence of nucleosomes from the LTP-promotor was not sufficient to provoke robust transcription in mutant atx1-leaf chromatin, suggesting that the mechanism repositioning nucleosomes at transition to flowering functioned independently of ATX1.

引用

页码：5199 / 5207

页数：9

共 30 条

[11] Dimethylation of histone H3 lysine 9 is a critical mark for DNA methylation and gene silencing in Arabidopsis thaliana [J].

Jackson, JP ;

Johnson, L ;

Jasencakova, Z ;

Zhang, X ;

PerezBurgos, L ;

Singh, PB ;

Cheng, XD ;

Schubert, I ;

Jenuwein, T ;

Jacobsen, SE .

CHROMOSOMA, 2004, 112 (06) :308-315

[12] Histone modifications in Arabidopsis -: high methylation of H3 lysine 9 is dispensable for constitutive heterochromatin [J].

Jasencakova, Z ;

Soppe, WJJ ;

Meister, A ;

Gernand, D ;

Turner, BM ;

Schubert, I .

PLANT JOURNAL, 2003, 33 (03) :471-480

[13] Mass spectrometry analysis of Arabidopsis histone H3 reveals distinct combinations of post-translational modifications [J].

Johnson, L ;

Mollah, S ;

Garcia, BA ;

Muratore, TL ;

Shabanowitz, J ;

Hunt, DF ;

Jacobsen, SE .

NUCLEIC ACIDS RESEARCH, 2004, 32 (22) :6511-6518

[14] Interplay between two epigenetic marks: DNA methylation and histone H3 lysine 9 methylation [J].

Johnson, LM ;

Cao, XF ;

Jacobsen, SE .

CURRENT BIOLOGY, 2002, 12 (16) :1360-1367

[15] Histone modifications defining active genes persist after transcriptional and mitotic inactivation [J].

Kouskouti, A ;

Talianidis, I .

EMBO JOURNAL, 2005, 24 (02) :347-357

[16] Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome [J].

Liang, GN ;

Lin, JCY ;

Wei, VV ;

Yoo, C ;

Cheng, JC ;

Nguyen, CT ;

Weisenberger, DJ ;

Egger, G ;

Takai, D ;

Gonzales, FA ;

Jones, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (19) :7357-7362

[17] Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3 [J].

Lindroth, AM ;

Shultis, D ;

Jasencakova, Z ;

Fuchs, J ;

Johnson, L ;

Schubert, D ;

Patnaik, D ;

Pradhan, S ;

Goodrich, J ;

Schubert, I ;

Jenuwein, T ;

Khorasanizadeh, S ;

Jacobsen, SE .

EMBO JOURNAL, 2004, 23 (21) :4146-4155

[18] Role of transposable elements in heterochromatin and epigenetic control [J].

Lippman, Z ;

Gendrel, AV ;

Black, M ;

Vaughn, MW ;

Dedhia, N ;

McCombie, WR ;

Lavine, K ;

Mittal, V ;

May, B ;

Kasschau, KD ;

Carrington, JC ;

Doerge, RW ;

Colot, V ;

Martienssen, R .

NATURE, 2004, 430 (6998) :471-476

[19] A plant dialect of the histone language [J].

Loidl, P .

TRENDS IN PLANT SCIENCE, 2004, 9 (02) :84-90

[20] Targeted recruitment of set1 histone methylase by elongating pol II provides a localized mark and memory of recent transcriptional activity [J].

Ng, HH ;

Robert, F ;

Young, RA ;

Struhl, K .

MOLECULAR CELL, 2003, 11 (03) :709-719

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