Presence of epsilon-adenosine tetraphosphate in chromaffin granules after transport of epsilon-ATP

被引：15

作者：

Gualix, J ^{[1
]}

Abal, M ^{[1
]}

Pintor, J ^{[1
]}

MirasPortugal, MT ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT BIOQUIM,E-28040 MADRID,SPAIN

来源：

FEBS LETTERS | 1996年 / 391卷 / 1-2期

关键词：

adenosine 5'-tetraphosphate; vesicular transport; etheno-adenine nucleotide; transphosphorylation; chromaffin granule;

D O I：

10.1016/0014-5793(96)00732-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adenosine 5'-tetraphosphate (Ap(4)) is a natural constituent of chromaffin granules with concentration values of 2.2 +/- 0.1 nmol/mg of protein and a ratio 245 +/- 40 times lower with respect to ATP (n = 4). The granular transport of epsilon-ATP resulted in a time- and concentration-dependent production of epsilon-adenosine tetraphosphate (epsilon-Ap(4)) at the intragranular level. The epsilon-Ap(4) formation followed a hyperbolic saturation kinetic at low epsilon-ATP concentrations with K-m value of 0.4 mu M epsilon-ATP intragranular (1.15 pmol/mg of granular protein). Intragranular concentrations of epsilon-ATP higher than 500 pmol/mg of protein (approximate to 175 mu M intragranular) resulted in a non-saturable production of epsilon-Ap(4).

引用

页码：195 / 198

页数：4

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