Characterization of pulsed plasma polymerization allylamine as an adhesion layer for DNA adsorption/hybridization

被引：90

作者：

Chen, Q ^{[1
]}

Förch, R

Knoll, W

机构：

[1] Chinese Acad Sci, Inst Plasma Phys, Beijing 100864, Peoples R China

[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

CHEMISTRY OF MATERIALS | 2004年 / 16卷 / 04期

关键词：

D O I：

10.1021/cm034529h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This paper presents an investigation of allylamine polymerization in pulsed radio frequency (RF) plasma as an adhesion layer immobilizing DNA probe for DNA hybridization assay. We looked for a simple and innovative way to improve the stability of pulsed plasma polymerization allylamine (PPPAA) film in phosphate-buffered saline (PBS) solution. To better understand the mechanism of PPPAA stability and the influence on DNA adsorption from films, several techniques were used. Fourier transform infrared (FTIR), atomic force microscopy (AFM), surface contact angle, and surface plasmon resonance (SPR) as well as surface plasmon enhanced fluorescence spectroscopy (SPFS) were all employed to investigate the effect of plasma conditions on the film structure, amine density, and the DNA hybridization reaction. It concludes that polymer deposited at low working pressure is very stable and can be used as adhesion layers for further study of DNA hybridization assay.

引用

页码：614 / 620

页数：7

共 29 条

[1] Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system
Affleck, DG
Yu, L
Bull, DA
Bailey, SH
Kim, SW
[J]. GENE THERAPY, 2001, 8 (05) : 349 - 353
[2] Agrawal Sudhir, 1995, Current Opinion in Biotechnology, V6, P12, DOI 10.1016/0958-1669(95)80003-4
[3] Deposition of highly ordered CF2-rich films using continuous wave and pulsed hexafluoropropylene oxide plasmas
Butoi, CI
Mackie, NM
Gamble, LJ
Castner, DG
Barnd, J
Miller, AM
Fisher, ER
[J]. CHEMISTRY OF MATERIALS, 2000, 12 (07) : 2014 - 2024
[4] Calderon JG, 1998, J BIOMED MATER RES, V42, P597, DOI 10.1002/(SICI)1097-4636(19981215)42:4<597::AID-JBM16>3.0.CO
[5] 2-R
[6] MOLECULAR-ORIGINS OF THE SLOW STREPTAVIDIN-BIOTIN DISSOCIATION KINETICS
CHILKOTI, A
STAYTON, PS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (43) : 10622 - 10628
[7] COOPER DN, 1993, HUM GENET, V92, P211
[8] CONTROL OF THE SPECIFIC ADSORPTION OF PROTEINS ONTO GOLD SURFACES WITH POLY(L-LYSINE) MONOLAYERS
FREY, BL
JORDAN, CE
KORNGUTH, S
CORN, RM
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (24) : 4452 - 4457
[9] Corona, spark and combined UV and ozone modification of polymer films WeBP23
Friedrich, J
Wigant, L
Unger, W
Lippitz, A
Wittrich, H
[J]. SURFACE & COATINGS TECHNOLOGY, 1998, 98 (1-3) : 879 - 885
[10] CHARACTERIZATION OF POLY-L-LYSINE ADSORPTION ONTO ALKANETHIOL-MODIFIED GOLD SURFACES WITH POLARIZATION-MODULATION FOURIER-TRANSFORM INFRARED-SPECTROSCOPY AND SURFACE-PLASMON RESONANCE MEASUREMENTS
JORDAN, CE
FREY, BL
KORNGUTH, S
CORN, RM
[J]. LANGMUIR, 1994, 10 (10) : 3642 - 3648

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