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Development and application of an electron spin resonance spectrometry method for the determination of oxygen free radical formation by particulate matter
被引:52
作者:
Briedé, JJ
De Kok, TMCM
Hogervorst, JGF
Moonen, EJC
Den Camp, CLBO
Kleinjans, JCS
机构:
[1] Univ Maastricht, Dept Hlth Risk Anal & Toxicol, NL-6200 MD Maastricht, Netherlands
[2] Univ Maastricht, Dept Gen Practice, NL-6200 MD Maastricht, Netherlands
关键词:
D O I:
10.1021/es0485311
中图分类号:
X [环境科学、安全科学];
学科分类号:
08 ;
0830 ;
摘要:
Exposure to increased levels of ambient particulate matter (PM) are associated with several health effects, including cardiopulmonary diseases. The formation of reactive oxygen species (ROS) is thought to play an important role in the induction of these health effects. To quantify the ROS generating capacity of PM,we developed an improved electron spin resonance (ESR) spectrometry-based method. ROS formation was measured directly on PM-containing filters, thereby avoiding the selective extraction of components and loss of material or reactivity, which is likely to occur during filter extraction. Also, ascorbic acid was added to stimulate ROS formation. This method was applied to PM10 samples originating from different sources. The radical generating capacity of PM10 from both gasoline and diesel engine exhaust was significantly higher as compared to that of PM10 from ambient or indoor air. Furthermore, in urban PM10 and PM2.5, ROS-generating capacity significantly correlated with concentrations of polycyclic aromatic hydrocarbon content and particular transition metals. This indicates that this improved ESR method may be a valuable tool for evaluating the relationship between RIDS formation by PM and the adverse health effects associated with this type of air pollution.
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页码:8420 / 8426
页数:7
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