Strategies for signal amplification in nucleic acid detection

被引：80

作者：

Andras, SC

Power, JB

Cocking, EC

Davey, MR

机构：

[1] Univ Nottingham, Sch Biosci, Plant Sci Div, Nottingham NG7 2RD, England

[2] Univ Babes Bolyai, Fac Biol & Geol, Dept Ecol & Genet, R-3400 Cluj Napoca, Romania

来源：

MOLECULAR BIOTECHNOLOGY | 2001年 / 19卷 / 01期

关键词：

signal amplification; nucleic acid detection; in situ hybridization; in situ PCR; primed in situ labeling; self-sustained sequence replication; strand displacement amplification; ligase chain reaction; padlock probes; rolling circle amplification; tyramide signal amplification; branched DNA amplification;

D O I：

10.1385/MB:19:1:029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction, Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification) are summarized in the present review, together with their advantages and limitations.

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页码：29 / 44

页数：16

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