Chlorine kinetic isotope effects on the haloalkane dehalogenase reaction

被引：46

作者：

Lewandowicz, A

Rudzinski, J

Tronstad, L

Widersten, M

Ryberg, P

Matsson, O

Paneth, P

机构：

[1] Tech Univ Lodz, Dept Chem, PL-90924 Lodz, Poland

[2] Uppsala Univ, Ctr Biomed, Dept Biochem, S-75123 Uppsala, Sweden

[3] Uppsala Univ, Dept Chem, S-75121 Uppsala, Sweden

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2001年 / 123卷 / 19期

关键词：

D O I：

10.1021/ja003503d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have found chlorine kinetic isotope effects on the dehalogenation catalyzed by haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 to be 1.0045 +/- 0.0004 fur 1,2-dichloroethane and 1.0066 +/- 0.0004 for 1-chlorobutane. The latter isotope effect approaches the intrinsic chlorine kinetic isotope effect for the dehalogenation step. The intrinsic isotope effect has been modeled using semiempirical and DFT theory levels using the ONIOM QM/QM scheme. Our results indicate that the dehalogenation step is reversible; the overall irreversibility of the enzyme-catalyzed reaction is brought about by a step following the dehalogenation.

引用

页码：4550 / 4555

页数：6

共 73 条

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