In vivo and in vitro effects of cadmium on H+ATPase activity of plasma membrane vesicles from oat (Avena sativa L.) roots

被引：34

作者：

Astolfi, S ^{[1
]}

Zuchi, S ^{[1
]}

Chiani, A ^{[1
]}

Passera, C ^{[1
]}

机构：

[1] Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2003年 / 160卷 / 04期

关键词：

Avena sativa L; cadmium; H(+)ATPase; plasma membrane; H+-ATPASE ACTIVITY; LIPID-COMPOSITION; INHIBITION; TOLERANCE; EXPRESSION; TOXICITY; FLUIDITY; METALS; NICKEL; RICE;

D O I：

10.1078/0176-1617-00832

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effect of an in vivo and in vitro treatment with cadmium on transport activities of root plasma membrane enriched vesicles was studied in oat (Avena sativa L. cv. Argentina) plants. Addition of 100 mumol/L CdSO4 to nutrient solution decreases both proton transport activity and ATPase activity to the same level. In vitro experiments show that cadmium seems to have a differential inhibiting effect on proton transport activity and ATPase activity, the most pronounced one on ATP-dependent H+-accumulation, suggesting that cadmium would interfere with membrane permeability properties. This is indeed the case. The results demonstrate that cadmium decreases passive permeability to protons.

引用

页码：387 / 393

页数：7

共 30 条

[1] Inducible expression of plasma membrane H+-ATPase in the roots of figleaf gourd plants under chilling root temperature [J].