Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass

被引:520
作者
García-Gil, JC [1 ]
Plaza, C [1 ]
Soler-Rovira, P [1 ]
Polo, A [1 ]
机构
[1] CSIC, Ctr Ciencias Medioambientales, E-28006 Madrid, Spain
关键词
soil biomass; enzyme activities; municipal solid waste compost; heavy metal pollution;
D O I
10.1016/S0038-0717(00)00165-6
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
A long-term field experiment utilising barley received four different treatments prior to sowing: municipal solid waste (MSW) compost at either 20 t ha(-1) (C20) or 80 t ha(-1) (C80); cow manure (MA) at 20 t ha(-1); mineral fertilizer (MIN) or NPK (400 kg ha(-1)); and NH4NO3 (150 kg ha(-1)). The effects of these applications on soil enzyme activities and microbial biomass at crop harvest were measured after nine years. In comparison with the control (no amendment) MSW addition increased biomass C by 10 and 46% at application rates of 20 and 80 t ha(-1), respectively, while MA treatment increased microbial biomass C by 29%. The ratio of soil microbial C to soil organic C was the lowest at the high rate of MSW application. Oxidoreductase enzymes, such as dehydrogenase and catalase, were higher in the MSW treatments by 730 (C20) and 200% (C80), respectively, and by 993 and 140% in MA treatments than in the unamended soil, indicating an increase in the microbial metabolism in the soil as a result of the mineralization of biodegradable C fractions contained in the amendments. The addition of MSW and MA caused different responses in hydrolase enzymes. Phosphatase activity decreased with MSW (+/- 62% at both rates) and MA (+/-73%), to less than those in the mineral fertilization and the control treatments. Urease activity decreased by 21% (C20) and 28% (C80), possibly being affected by the heavy metals contained in the MSW. However, beta -glucosidase and protease-BAA increased in all the organic treatments, especially with MA (by 214 and 177%, respectively). This is attributed to the microbial stimulation by the organic C and is correlated with the increase in dehydrogenase (r(2) = 0.882) and catalase (r(2) = 0.654) activities. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1907 / 1913
页数:7
相关论文
共 42 条
[21]   APPLICATION OF THE BERTHELOT REACTION TO THE DETERMINATION OF AMMONIUM-N IN SOIL EXTRACTS AND SOIL DIGESTS [J].
HINDS, AA ;
LOWE, LE .
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 1980, 11 (05) :469-475
[22]   IMPACT OF AGRICULTURAL PRACTICES ON THE SIZE AND ACTIVITY OF THE MICROBIAL BIOMASS IN A LONG-TERM FIELD EXPERIMENT [J].
HOUOT, S ;
CHAUSSOD, R .
BIOLOGY AND FERTILITY OF SOILS, 1995, 19 (04) :309-316
[23]   DIE BILDUNG VON UREASE NACH VERBRAUCH DER AUSSEREN N-QUELLE BEI HYDROGENOMONAS H16 [J].
KONIG, C ;
KALTWASSER, H ;
SCHLEGEL, HG .
ARCHIV FUR MIKROBIOLOGIE, 1966, 53 (03) :231-+
[24]  
LADD JN, 1978, SOIL ENZYMES, P51
[25]  
Marzadori C, 1996, BIOL FERT SOILS, V22, P53, DOI 10.1007/BF00384432
[26]  
MASCIANDARO G, 1994, AGROCHIMICA, V38, P195
[27]   CHANGES IN AMINO-ACIDS, ENZYME-ACTIVITIES, AND BIOMASSES DURING SOIL MICROBIAL-GROWTH [J].
NANNIPIERI, P ;
PEDRAZZINI, F ;
ARCARA, PG ;
PIOVANELLI, C .
SOIL SCIENCE, 1979, 127 (01) :26-34
[28]   EXTRACTION OF PHOSPHATASE, UREASE, PROTEASES, ORGANIC-CARBON, AND NITROGEN FROM SOIL [J].
NANNIPIERI, P ;
CECCANTI, B ;
CERVELLI, S ;
MATARESE, E .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1980, 44 (05) :1011-1016
[29]  
Nannipieri P., 1994, Soil biota: management in sustainable farming systems., P238
[30]   ENZYME-ACTIVITY AND MICROBIAL BIOMASS IN A FIELD SOIL AMENDED WITH MUNICIPAL REFUSE [J].
PERUCCI, P .
BIOLOGY AND FERTILITY OF SOILS, 1992, 14 (01) :54-60