Reduced IP3 sensitivity of IP3 receptor in Purkinje neurons

被引：25

作者：

Fujiwara, A

Hirose, K

Yamazawa, T

Lino, M ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Pharmacol, Tokyo 1130033, Japan

[2] Japan Sci & Technol Corp, CREST, Tokyo 1130033, Japan

来源：

NEUROREPORT | 2001年 / 12卷 / 12期

关键词：

Ca2+; Ca2+ imaging; IP3; receptor; long-term depression; permeabilization; Purkinje neuron;

D O I：

10.1097/00001756-200108280-00012

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The inositol 1,4,S-trisphosphate receptor (IP3R) is highly expressed in Purkinje neurons (PNs) and is thought to be essential for the induction of long-term depression at parallel-fiber-PN synapses. Here, by imaging the fluorescence intensity of the low-affinity Ca2+ indicator inside the Ca2+ stores in the permeabilized single PNs, we analyzed the kinetics of Ca2+ release via the IP3R in controlled cytoplasmic environments. The rate of Ca2+ release is dependent on the IP3 concentration with an EC50 of 25.8 muM, which is > 20-fold greater than that of the IP3R in the isolated preparations or in peripheral cells. This property would be advantageous in inducing the release of Ca2+ in a localized space adjacent to the site of synaptic inputs. NeuroReport 12:2647-2651 (C) 2001 Lippincott Williams & Wilkins.

引用

页码：2647 / 2651

页数：5

共 25 条

[1] REDUCED HIPPOCAMPAL LONG-TERM POTENTIATION AND CONTEXT-SPECIFIC DEFICIT IN ASSOCIATIVE LEARNING IN MGLUR1 MUTANT MICE [J].