Reduced IP3 sensitivity of IP3 receptor in Purkinje neurons

被引：25

作者：

Fujiwara, A

Hirose, K

Yamazawa, T

Lino, M ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Pharmacol, Tokyo 1130033, Japan

[2] Japan Sci & Technol Corp, CREST, Tokyo 1130033, Japan

来源：

NEUROREPORT | 2001年 / 12卷 / 12期

关键词：

Ca2+; Ca2+ imaging; IP3; receptor; long-term depression; permeabilization; Purkinje neuron;

D O I：

10.1097/00001756-200108280-00012

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The inositol 1,4,S-trisphosphate receptor (IP3R) is highly expressed in Purkinje neurons (PNs) and is thought to be essential for the induction of long-term depression at parallel-fiber-PN synapses. Here, by imaging the fluorescence intensity of the low-affinity Ca2+ indicator inside the Ca2+ stores in the permeabilized single PNs, we analyzed the kinetics of Ca2+ release via the IP3R in controlled cytoplasmic environments. The rate of Ca2+ release is dependent on the IP3 concentration with an EC50 of 25.8 muM, which is > 20-fold greater than that of the IP3R in the isolated preparations or in peripheral cells. This property would be advantageous in inducing the release of Ca2+ in a localized space adjacent to the site of synaptic inputs. NeuroReport 12:2647-2651 (C) 2001 Lippincott Williams & Wilkins.

引用

收藏

页码：2647 / 2651

页数：5

相关论文

共 25 条

[11] SPATIAL DYNAMICS OF 2ND MESSENGERS - IP3 AND CAMP AS LONG-RANGE AND ASSOCIATIVE MESSENGERS [J].

KASAI, H ;

PETERSEN, OH .

TRENDS IN NEUROSCIENCES, 1994, 17 (03) :95-101

[12] FAST ACTIVATION AND INACTIVATION OF INOSITOL TRISPHOSPHATE-EVOKED CA2+ RELEASE IN RAT CEREBELLAR PURKINJE NEURONS [J].

KHODAKHAH, K ;

OGDEN, D .

JOURNAL OF PHYSIOLOGY-LONDON, 1995, 487 (02) :343-358

[13] FUNCTIONAL-HETEROGENEITY OF CALCIUM-RELEASE BY INOSITOL TRISPHOSPHATE IN SINGLE PURKINJE NEURONS, CULTURED CEREBELLAR ASTROCYTES, AND PERIPHERAL-TISSUES [J].

KHODAKHAH, K ;

OGDEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) :4976-4980

[14] BRIEF DENDRITIC CALCIUM SIGNALS INITIATE LONG-LASTING SYNAPTIC DEPRESSION IN CEREBELLAR PURKINJE-CELLS [J].

KONNERTH, A ;

DREESSEN, J ;

AUGUSTINE, GJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7051-7055

[15] Functional coupling of phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate receptor [J].

Lupu, VD ;

Kaznacheyeva, E ;

Krishna, UM ;

Falck, JR ;

Bezprozvanny, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (23) :14067-14070

[16] Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor [J].

Matsumoto, M ;

Nakagawa, T ;

Inoue, T ;

Nagata, E ;

Tanaka, K ;

Takano, H ;

Minowa, O ;

Kuno, J ;

Sakakibara, S ;

Yamada, M ;

Yoneshima, H ;

Miyawaki, A ;

Fukuuchi, Y ;

Furuichi, T ;

Okano, H ;

Mikoshiba, K ;

Noda, T .

NATURE, 1996, 379 (6561) :168-171

[17] The bell-shaped Ca2+ dependence of the inositol 1,4,5-trisphosphate-induced Ca2+ release is modulated by Ca2+/calmodulin [J].

Missiaen, L ;

Parys, JB ;

Weidema, AF ;

Sipma, H ;

Vanlingen, S ;

De Smet, P ;

Callewaert, G ;

De Smedt, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (20) :13748-13751

[18] Ca2+-sensor region of IP3 receptor controls intracellular Ca2+ signaling [J].

Miyakawa, T ;

Mizushima, A ;

Hirose, K ;

Yamazawa, T ;

Bezprozvanny, I ;

Kurosaki, T ;

Iino, M .

EMBO JOURNAL, 2001, 20 (07) :1674-1680

[19] Local calcium release in dendritic spines required for long-term synaptic depression [J].

Miyata, M ;

Finch, EA ;

Khiroug, L ;

Hashimoto, K ;

Hayasaka, S ;

Oda, SI ;

Inouye, M ;

Takagishi, Y ;

Augustine, GJ ;

Kano, M .

NEURON, 2000, 28 (01) :233-244

[20] MAPPING CALCIUM TRANSIENTS IN THE DENDRITES OF PURKINJE-CELLS FROM THE GUINEA-PIG CEREBELLUM INVITRO [J].

ROSS, WN ;

WERMAN, R .

JOURNAL OF PHYSIOLOGY-LONDON, 1987, 389 :319-336