Genetic optimization of recombinant glycoprotein production by mammalian cells

被引：89

作者：

Fussenegger, M ^{[1
]}

Bailey, JE

Hauser, H

Mueller, PP

机构：

[1] ETH Honggerberg, Inst Biotechnol, HPT, CH-8093 Zurich, Switzerland

[2] GBF, Natl Res Ctr Biotechnol, Dept Gene Regulat & Differentiat, D-38124 Braunschweig, Germany

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 01期

关键词：

D O I：

10.1016/S0167-7799(98)01248-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetically modified mammalian cells are the preferred system for the production of recombinant therapeutic glycoproteins. Other applications include engineering of cell lines for drug screening and cell-based therapies, and the construction of recombinant viruses for gene therapy. This article highlights contemporary core genetic technologies and emerging strategies for genetically engineering mammalian cells for optimal recombinant-protein expression.

引用

页码：35 / 42

页数：8

共 124 条

[1] SPECIFIC MONOCLONAL-ANTIBODY PRODUCTIVITY AND THE CELL CYCLE-COMPARISONS OF BATCH, CONTINUOUS AND PERFUSION CULTURES [J].

ALRUBEAI, M ;

EMERY, AN ;

CHALDER, S ;

JAN, DC .

CYTOTECHNOLOGY, 1992, 9 (1-3) :85-97

[2] DEATH MECHANISMS OF ANIMAL-CELLS IN CONDITIONS OF INTENSIVE AGITATION [J].

ALRUBEAI, M ;

SINGH, RP ;

GOLDMAN, MH ;

EMERY, AN .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 45 (06) :463-472

[3]

Bailey JE, 1997, ANIMAL CELL TECHNOLOGY, P489

[4] TOWARD A SCIENCE OF METABOLIC ENGINEERING [J].

BAILEY, JE .

SCIENCE, 1991, 252 (5013) :1668-1675

[5]

BAILEY JE, 1998, NEW DEV NEW APPL ANI, P5

[6] COREGULATION OF 2 GENE ACTIVITIES BY TETRACYCLINE VIA A BIDIRECTIONAL PROMOTER [J].

BARON, U ;

FREUNDLIEB, S ;

GOSSEN, M ;

BUJARD, H .

NUCLEIC ACIDS RESEARCH, 1995, 23 (17) :3605-3606

[7] SEMLIKI FOREST VIRUS EXPRESSION SYSTEM - PRODUCTION OF CONDITIONALLY INFECTIOUS RECOMBINANT PARTICLES [J].

BERGLUND, P ;

SJOBERG, M ;

GAROFF, H ;

ATKINS, GJ ;

SHEAHAN, BJ ;

LILJESTROM, P .

BIO-TECHNOLOGY, 1993, 11 (08) :916-920

[8] Scaffold/matrix-attached regions: Topological switches with multiple regulatory functions [J].

Bode, J ;

StengertIber, M ;

Kay, V ;

Schlake, T ;

DietzPfeilstetter, A .

CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION, 1996, 6 (2-3) :115-138

[9]

Bode J, 1995, INT REV CYTOL, V162A, P389

[10] Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins [J].

Borman, AM ;

LeMercier, P ;

Girard, M ;

Kean, KM .

NUCLEIC ACIDS RESEARCH, 1997, 25 (05) :925-932

← 1 2 3 4 5 6 7 8 9 10 →