Genetic optimization of recombinant glycoprotein production by mammalian cells

被引：89

作者：

Fussenegger, M ^{[1
]}

Bailey, JE

Hauser, H

Mueller, PP

机构：

[1] ETH Honggerberg, Inst Biotechnol, HPT, CH-8093 Zurich, Switzerland

[2] GBF, Natl Res Ctr Biotechnol, Dept Gene Regulat & Differentiat, D-38124 Braunschweig, Germany

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 01期

关键词：

D O I：

10.1016/S0167-7799(98)01248-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetically modified mammalian cells are the preferred system for the production of recombinant therapeutic glycoproteins. Other applications include engineering of cell lines for drug screening and cell-based therapies, and the construction of recombinant viruses for gene therapy. This article highlights contemporary core genetic technologies and emerging strategies for genetically engineering mammalian cells for optimal recombinant-protein expression.

引用

页码：35 / 42

页数：8

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