Intracellular FITC-derivatization with PEG

被引：4

作者：

Chen, FG ^{[1
]}

Zhi, DY ^{[1
]}

Xia, GM ^{[1
]}

机构：

[1] Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 21期

关键词：

capillary electrophoresis; fluorescein isothiocyanate; free amino acids; intracellular derivatization; laser-induced fluorescence; polyethylene glycol; protoplasts of wheat embryonic calli;

D O I：

10.1002/elps.200410300

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to investigate the amino acids (AAs) in plant cells, we explore an avenue for intracellular derivatization with FITC. In this method, FITC was used to mark AAs in living protoplasts derived from embryogenic calli of common wheat (Triticum aestivum L. c.v. Jinan 177) mediated by PEG. After FITC-derivatization, the AAs; in the lysate were determined by CE. The result reveals that this PEG method can be used to transfer FITC into plant cells efficiently, which provides a good method for AA analysis in plant cells.

引用

页码：4204 / 4205

页数：2

共 13 条

[1] Chemical profiles and monitoring dynamics at an individual nerve cell in Planorbis corneus with electrochemical detection
Anderson, BB
Ewing, AG
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 19 (1-2) : 15 - 32
[2] Dermaux A, 1999, ELECTROPHORESIS, V20, P3027, DOI 10.1002/(SICI)1522-2683(19991001)20:15/16<3027::AID-ELPS3027>3.0.CO
[3] 2-Z
[4] ANALYSIS OF SINGLE CELLS BY CAPILLARY ELECTROPHORESIS WITH ON COLUMN DERIVATIZATION AND LASER-INDUCED FLUORESCENCE DETECTION
GILMAN, SD
EWING, AG
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (01) : 58 - 64
[5] GU H, 2003, PANT GENES MOL MANIP, P251
[6] GUO GQ, 1991, SCI CHINA SER B, V34, P438
[7] MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS
KENNEDY, RT
OATES, MD
COOPER, BR
NICKERSON, B
JORGENSON, JW
[J]. SCIENCE, 1989, 246 (4926) : 57 - 63
[8] BALL-LENS LASER-INDUCED FLUORESCENCE DETECTOR AS AN EASY-TO-USE HIGHLY SENSITIVE DETECTOR FOR CAPILLARY ELECTROPHORESIS - APPLICATION TO THE IDENTIFICATION OF BIOGENIC-AMINES IN DAIRY-PRODUCTS
NOUADJE, G
NERTZ, M
VERDEGUER, P
COUDERC, F
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 717 (1-2) : 335 - 343
[9] CHILD CEREBROSPINAL-FLUID ANALYSIS BY CAPILLARY ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE DETECTION
NOUADJE, G
RUBIE, H
CHATELUT, E
CANAL, P
NERTZ, M
PUIG, P
COUDERC, F
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 717 (1-2) : 293 - 298
[10] QUANTITATIVE AMINO-ACID-ANALYSIS OF INDIVIDUAL SNAIL NEURONS BY OPEN TUBULAR LIQUID-CHROMATOGRAPHY
OATES, MD
COOPER, BR
JORGENSON, JW
[J]. ANALYTICAL CHEMISTRY, 1990, 62 (15) : 1573 - 1577

← 1 2 →