Mouse fibroblast expressing human tyrosinase with DHICA-oxidase activity produces predominantly pheomelanin deposit in lysosome

被引：14

作者：

Kondoh, H ^{[1
]}

Wilczek, A ^{[1
]}

Narimizu, S ^{[1
]}

Mishima, Y ^{[1
]}

机构：

[1] SANSYO PHARMACEUT CO LTD,OHNOJYO SHI 816,JAPAN

来源：

ZOOLOGICAL SCIENCE | 1996年 / 13卷 / 06期

关键词：

D O I：

10.2108/zsj.13.825

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The melanogenic gene-transfected cell system serves as a useful tool for the study of the symphonic relation between melanin synthesis and intracellular organelles such as melanosomes in melanocytes. We constructed melanin-producing mouse fibroblasts by transfection of human tyrosinase cDNA to investigate the intracellular changes caused by tyrosinase expression. DHICA-oxidase (5,6-dihydroxyindole-2-carboxylic acid oxidase) activity without TRP-1 (Tyrosinase Related Protein-1) expression in the cells suggested that human tyrosinase also possesses a DHICA-oxidase activities different from mouse tyrosinase. Electron microscopic observation indicated that melanin-deposit organelles have some lysosomal features. These properties of melanin-deposit organelles in tyrosinase expressing fibroblasts provide one evidence for the hypothesis that melanosome is the specialized lysosome in melanocytes.

引用

页码：825 / 831

页数：7

共 40 条

[21] MELANOSOMES ARE SPECIALIZED MEMBERS OF THE LYSOSOMAL LINEAGE OF ORGANELLES [J].