Bm-TFF2, a toad trefoil factor, promotes cell migration, survival and wound healing

被引：39

作者：

Zhang, Yong ^{[1
,2
,3
]}

Yu, Guoyu ^{[1
,2
,3
,4
]}

Xiang, Yang ^{[1
,2
,3
]}

Wu, Jianbo ^{[1
,2
]}

Jiang, Ping ^{[1
,2
,3
]}

Lee, Wenhui ^{[1
,2
]}

Zhang, Yun ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China

[2] Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

[4] Kunming Med Coll, Dept Biochem, Kunming 650032, Yunnan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 398卷 / 03期

关键词：

Amphibian; Skin; Trefoil factor; Cell migration; Wound healing; Anti-apoptosis; BETA-GAMMA-CRYSTALLIN; BOMBINA-MAXIMA SKIN; BREAST-CANCER CELLS; FACTOR COMPLEX; AMPHIBIAN SKIN; GROWTH-FACTOR; SPASMOLYTIC POLYPEPTIDE; PROTEIN; SECRETIONS; STOMACH;

D O I：

10.1016/j.bbrc.2010.06.118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Toad skin is naked and continually confronted by various injurious factors. Constant skin renewal and repairs occur frequently. However, the mechanisms of the renewal and repair have not clearly elucidated. In our previous work, a trefoil factor (TFF), Bm-TFF2, has been purified from the Bombina maxima skin and characterized as a platelet agonist. The mRNA of TFFs in toad skin was up-regulated greatly during the metamorphosis, indicating a pivotal role of TFFs in amphibian skin. Here, we presented the effects of Bm-TFF2 on the cell migration, apoptosis and proliferation. Bm-TFF2 bound to epithelial cells and showed strong cell motility activity. At the concentrations of 1-100 nM, Bm-TFF2-induced migration of human epithelial AGS and HT-29 cells, and rat intestinal epithelial IEC-6 cell lines. The in vitro wound healing assay also verified the activity of Bm-TFF2. Bm-TFF2 could also inhibit cell apoptosis induced by ceramide and sodium butyrate. The cell migration-promoting activity was abolished by MEK1 inhibitors, U0126 and PD98059, suggesting that ERK1/2 activation is crucial for Bm-TFF2 to stimulate cell migration. Taken together, Bm-TFF2 promoted wound healing by stimulating cell migration via MAPK pathway and preventing cell apoptosis. The potent biological activity of Bm-TFF2 makes it a useful molecular tool for further studies of structure-function relationship of the related human TFFs. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：559 / 564

页数：6

共 32 条

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