Root-associated fungal microbiota of nonmycorrhizal &ITArabis&IT &ITalpina &ITand its contribution to plant phosphorus nutrition

被引:200
作者
Almario, Juliana [1 ,2 ,4 ]
Jeena, Ganga [1 ,2 ]
Wunder, Joerg [3 ]
Langen, Gregor [1 ,2 ]
Zuccaro, Alga [1 ,2 ]
Coupland, George [2 ,3 ]
Bucher, Marcel [1 ,2 ]
机构
[1] Univ Cologne, Cologne Bioctr, Bot Inst, D-50674 Cologne, Germany
[2] Univ Cologne, Cluster Excellence Plant Sci, D-50674 Cologne, Germany
[3] Max Planck Inst Plant Breeding Res, Dept Plant Dev Biol, D-50829 Cologne, Germany
[4] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
关键词
Brassicaceae; microbiome; fungal endophyte; Helotiales; nutrient transfer; RHIZOSPHERE MICROBIOME; SEQUENCE ALIGNMENT; ENDOPHYTIC FUNGUS; ARABIS-ALPINA; PHOSPHATE; ECOLOGY; BIOGEOGRAPHY; DIVERSITY; TRANSPORT; SYMBIOSIS;
D O I
10.1073/pnas.1710455114
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Most land plants live in association with arbuscular mycorrhizal (AM) fungi and rely on this symbiosis to scavenge phosphorus (P) from soil. The ability to establish this partnership has been lost in some plant lineages like the Brassicaceae, which raises the question of what alternative nutrition strategies such plants have to grow in P-impoverished soils. To understand the contribution of plant-micro biota interactions, we studied the root-associated fungal microbiome of Arabis alpina (Brassicaceae) with the hypothesis that some of its components can promote plant P acquisition. Using amplicon sequenc ing of the fungal internal transcribed spacer 2, we studied the root and rhizosphere fungal communities of A. alpina growing under natural and controlled conditions including low-P soils and identified a set of 15 fungal taxa consistently detected in its roots. This cohort included a Helotiales taxon exhibiting high abundance in roots of wild A. alpina growing in an extremely P-limited soil. Consequently, we isolated and subsequently reintroduced a specimen from this taxon into its native P-poor soil in which it improved plant growth and P uptake. The fungus exhibited mycorrhiza-like traits including colonization of the root endosphere and P transfer to the plant Genome analysis revealed a link between its endophytic lifestyle and the expansion of its repertoire of carbohydrate-active enzymes. We report the discovery of a plant- fungus interaction facilitating the growth of a nonmycorrhizal plant under native P-limited conditions, thus uncovering a previously underestimated role of root fungal microbiota in P cycling.
引用
收藏
页码:E9403 / E9412
页数:10
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