Isolation of human mesenchymal stem cells from bone and adipose tissue

The above protocols are intended to provide practical, generally applicable methods for isolating hMSCs from different tissues. They have been used successfully by the authors to prepare and characterize hMSCs from over 50 bone and adipose tissue samples over 3 years. However, the protocols should be considered as guidelines, and a starting point for researchers interested in preparing hMSCs for their own applications. Investigators should not hesitate to modify the protocols to correspond with ongoing research projects, individual laboratory practices, or to use available equipment or supplies. The authors have not attempted to isolate MSCs from nonhuman animals, however, minor modifications, for example, in the composition of media, may allow these protocols to be used successfully with other species. © 2008 Elsevier Inc. All rights reserved.