Improved recovery of rabies virus from cloned cDNA using a vaccinia virus-free reverse genetics system

被引：181

作者：

Ito, N ^{[1
]}

Takayama-Ito, M ^{[1
]}

Yamada, K ^{[1
]}

Hosokawa, J ^{[1
]}

Sugiyama, M ^{[1
]}

Minamoto, N ^{[1
]}

机构：

[1] Gifu Univ, Fac Agr, Lab Zoonot Dis, Div Vet Med, Gifu 5011193, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 47卷 / 08期

关键词：

rabies virus; reverse genetics; T7 RNA polymerase-expressing BHK cells; internal ribosomal entry site;

D O I：

10.1111/j.1348-0421.2003.tb03424.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To improve efficiency of recovery of rabies virus from cloned cDNA, we established a BHK cell clone that stably expresses T7 RNA polymerase, which we named BHK/T7-9. We also constructed new helper plasmids for expression of nucleoprotein and RNA polymerase of the RC-HL strain using the pTM1 plasmid vector, which makes the T7 RNA polymerase-transcripts from the plasmid cap-independent for translation. After co-transfection of these helper plasmids and the previously constructed full-length genome plasmid of the RC-HL strain to BHK/T7-9 cells, recombinant rabies virus was efficiently recovered from the cloned cDNA.

引用

页码：613 / 617

页数：5

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