Microchip separation and electrochemical detection of amino acids and peptides following precolumn derivatization with naphthalene-2,3-dicarboxyaldehyde

被引:37
作者
Wang, J [1 ]
Chen, G [1 ]
Pumera, M [1 ]
机构
[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA
关键词
amino acids; peptides; microchips; capillary electrophoresis; amperometric detection; naphthalene-2,3-dicarboxyaldehyde;
D O I
10.1002/elan.200390106
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene-2,3-dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 muM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end-column thick-film carbon-electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on-chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.
引用
收藏
页码:862 / 865
页数:4
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