Chitosan-poly (glycidyl methacrylate) copolymer for immobilization of urease

被引：65

作者：

Chellapandian, M ^{[1
]}

Krishnan, MRV ^{[1
]}

机构：

[1] Anna Univ, Dept Chem Engn, Madras 600025, Tamil Nadu, India

来源：

PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 06期

关键词：

immobilization; urease; chitosan; chitosan-poly (GMA);

D O I：

10.1016/S0032-9592(98)80001-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Urease was covalently attached onto chitosan-poly (GMA) by the introduction of epoxy groups to the support. The immobilized urease retained 82.15% of its specific activity. The pH optimum of the free and immobilized urease was unchanged, but the optimum temperature of the immobilized urease was higher than that of the free enzyme. Immobilization improved the thermal, pH and storage stability of the enzyme. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：595 / 600

页数：6

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