Metazoan Operons Accelerate Recovery from Growth-Arrested States

被引：37

作者：

Zaslaver, Alon ^{[1
,2
]}

Baugh, L. Ryan ^{[3
,4
]}

Sternberg, Paul W. ^{[1
,2
]}

机构：

[1] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

[3] Duke Univ, Dept Biol, Durham, NC 27708 USA

[4] Duke Univ, IGSP Ctr Syst Biol, Durham, NC 27708 USA

来源：

CELL | 2011年 / 145卷 / 06期

关键词：

ELEGANS DAUER LARVA; CAENORHABDITIS-ELEGANS; GENE-EXPRESSION; GLOBAL ANALYSIS; GENOME; ORGANIZATION; PROMOTERS; DYNAMICS; SEQUENCE; CHORDATE;

D O I：

10.1016/j.cell.2011.05.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Existing theories explain why operons are advantageous in prokaryotes, but their occurrence in metazoans is an enigma. Nematode operon genes, typically consisting of growth genes, are significantly upregulated during recovery from growth-arrested states. This expression pattern is anticorrelated to nonoperon genes, consistent with a competition for transcriptional resources. We find that transcriptional resources are initially limiting during recovery and that recovering animals are highly sensitive to any additional decrease in transcriptional resources. We provide evidence that operons become advantageous because, by clustering growth genes into operons, fewer promoters compete for the limited transcriptional machinery, effectively increasing the concentration of transcriptional resources and accelerating recovery. Mathematical modeling reveals how a moderate increase in transcriptional resources can substantially enhance transcription rate and recovery. This design principle occurs in different nematodes and the chordate C. intestinalis. As transition from arrest to rapid growth is shared by many metazoans, operons could have evolved to facilitate these processes.

引用

页码：981 / 992

页数：12

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