Direct binding to nucleic acids by Vpr of human immunodeficiency virus type 1

被引:53
作者
Zhang, SG
Pointer, D
Singer, G
Feng, YF
Park, K
Zhao, LJ
机构
[1] St Louis Univ, Sch Med, Inst Mol Virol, St Louis, MO 63110 USA
[2] Univ Kansas, Dept Microbiol, Lawrence, KS 66045 USA
关键词
HIV; Vpr; DNA; RNA; interaction;
D O I
10.1016/S0378-1119(98)00178-4
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) is a 15 kDa regulatory protein packaged in the HIV-1 virion. Although the molecular mechanism of Vpr function during viral replication remains elusive, Vpr has been found to possess interesting biological activities, including cell-cycle arrest at the G2/M check point, promotion of the HIV-1 pre-integration complex for nuclear transport, and a low but significant level of transcriptional activation of a variety of viral and cellular promoters. We now present data suggesting that HIV-1 Vpr is a nucleic-acid-binding protein. This activity of Vpr was demonstrated by DNA-cellulose chromatography, antibody co-immunoprecipitation, and gel electrophoretic mobility shift assays. By mutational analysis, the C-terminal region of Vpr, which is rich in basic amino-acid residues, was shown to be critical for Vpr binding to nucleic acids. The nucleic-acid-binding activity of Vpr is consistent with several biological activities of Vpr and may provide an important clue for understanding the molecular interactions between HIV-1 and the host cells. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:157 / 166
页数:10
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