Highly-parallel metabolomics approaches using LC-MS2 for pharmaceutical and environmental analysis

被引：35

作者：

Bajad, Sunil ^{[1
]}

Shulaev, Vladimir ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Bioinformat Inst, Blacksburg, VA 24061 USA

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2007年 / 26卷 / 06期

关键词：

chromatography; environmental; LC-MS2; metabolomics; pharmaceutical;

D O I：

10.1016/j.trac.2007.02.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The 'omics' approaches - genomics, proteomics and metabolomics - are based on high-throughput, high-information-content analysis. Using these approaches, as opposed to targeting one or a few analytes, a holistic understanding of the composition of a sample can be obtained. These approaches have revolutionized sample-analysis and data-processing protocols. In metabolomic studies, hundreds of small molecules are simultaneously analyzed using analytical platforms (e.g., gas chromatography-mass spectrometry (GC-MS) or liquid chromatography coupled to tandem mass spectrometry (LC-MS')). This philosophy of holistic analysis and the application of high-throughput, high-information-content analysis offer several advantages. In this article, we compare the conventional analytical approach of one (or a few) analyte(s) per sample to the LC-MS2-based metabolomic-type approach in the context of pharmaceutical and environmental analysis. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：625 / 636

页数：12

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