Preparation of a polymethoxyflavone-rich fraction (PRF) of Citrus sunki Hort. ex Tanaka and its antiproliferative effects

被引：36

作者：

Ko, Hee-Chul ^{[3
]}

Jang, Mi-Gyeong ^{[1
,2
]}

Kang, Chang-Hee ^{[3
]}

Lee, Nam-Ho ^{[3
]}

Kang, Seong-Il ^{[1
,2
]}

Lee, Sun-Ryung ^{[1
,2
]}

Park, Deok-Bae ^{[4
]}

Kim, Se-Jae ^{[1
,2
]}

机构：

[1] Jeju Natl Univ, Dept Biol, Cheju 690756, South Korea

[2] Jeju Natl Univ, Res Inst Basic Sci, Cheju 690756, South Korea

[3] Jeju Natl Univ, Dept Chem, Cheju 690756, South Korea

[4] Jeju Natl Univ, Sch Med, Cheju 690756, South Korea

来源：

FOOD CHEMISTRY | 2010年 / 123卷 / 02期

关键词：

Citrus sunki Hort. ex Tanaka; Polymethoxyflavone; PMF-rich fraction (PRF); Antiproliferative activity; POLYMETHOXYLATED FLAVONES; NOBILETIN; INHIBITORS; APOPTOSIS; EXTRACTS;

D O I：

10.1016/j.foodchem.2010.04.028

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The peel of Citrus sunki is a rich source of polymethoxyflavones (PMFs). In this study, a PMF-rich fraction (PRF) was obtained from the peels of C sunki by hot-water extraction and subsequent fractionation with n-hexane. Six PMFs and two demethylated PMFs were isolated by semi-preparative HPLC, and their structures were identified by H-1 NMR and C-13 NMR. To define the synergistic effects of PRF, the PRF and its eight individual components were assessed for antiproliferative activity using HL-60 cells. The PRF showed greater growth inhibitory activity (IC50 value = 25.14 mu g/ml), compared with its individual components. Flow cytometric analysis confirmed that the PRF significantly increased the percentage of sub-G(1) cells (30.1% at 25 mu g/ml). Our study provides an efficient method for preparing a PRF for use as a functional food ingredient. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：484 / 488

页数：5

共 24 条

[1]

Sinensetin, eupatorin, 3′-hydroxy-5,6,7,4′-tetramethoxyflavone and rosmarinic acid contents and antioxidative effect of Orthosiphon stamineus from Malaysia [J].